„One Health“ koncept u prevenciji pojave ostataka β-laktamskih antibiotika u mlijeku

master's thesis

• Title (english): Preventing β-lactam antibiotic residues appearance in milk by "One Health" approach
• Author: Ana Širić
• Mentor: Blaženka Kos (mentor)
• Committee member: Jagoda Šušković (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2020-12-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Pretjerana uporaba antibiotika u terapijama te kao promotora rasta životinja na farmama uzrokovala je naglo širenje antibiotičke rezistencije među mikroorganizmima. Također, javlja se problem ostataka antibiotika u životinjskoj hrani, koju konzumira čovjek, kao i u okolišu, gdje dolazi do zagađenja tla i vode. Za siguran život ljudi i životinja te očuvanje okoliša, nužan je „One Health“ koncept koji podrazumijeva sveobuhvatnu brigu za zdravlje ljudi, životinja i ekosustava te njegova primjena ima potencijala u globalnom poboljšanju uvjeta života na Zemlji. Cilj ovog rada je bio ispitivanje i identifikacija ostataka β-laktamskih antibiotika u uzorcima mlijeka, kao i razvoj metode i ispitivanje utjecaja metode za identifikaciju rezidua β-laktama u kravljem i kozjem mlijeku primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS). Primjenom ove metode utvrđen je jedan pozitivni uzorak na rezidue amoksicilina pri koncentraciji većoj od najveće dopuštene količine (NDK) utvrđene Uredbom Komisije (EU) (2010) br. 37/2010.

Abstract (english)

Excessive ussage of antibiotics in therapies and as animal growth factors on farms, cause sudden spread antibiotic resistance among microorganisms. Besides that, problem is not only with antibiotic residues in animal food which human consumes, but also in environment, where soil and water pollution occurs . „One Health“ approach is necessary for safe human and animal life and environmental protection and it includes human, animal and environment health care. Its appliance has potential in global improvement of life on Earth. The objective of this paper was to identify and quantify β-lactams residues in milk samples as well as to develop a method and to examine the effect of the method on β-lactam residues determination in goat's and cow's milk using ultra-high performance liquid chromatography and high resolution mass spectrometry (UHPLC/Q-TOF-MS) Using this method, one positive sample was found for amoxicillin residues at a concentration higher than the maximum residue level (MRL) prescribed by the European Commission Regulation (EC) no. 37/2010.

• Keywords: „One Health“; ostaci β-laktamskih antibiotika; UHLPC/Q-TOF-MS
• Keywords (english): „One Health“ approach; β-lactam antibiotics residues; UHPLC/Q-TOF-MS
• Language: croatian
• URN:NBN: urn:nbn:hr:159:319900
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-01-13 10:23:44