Doprinos prirodnih eutektičkih otapala u valorizaciji otpadne kore naranče

master's thesis

• Title (english): Natural deep eutectic solvents contribution in orange peel waste valorization
• Author: Lucija Jaša-Šangulin
• Mentor: Marina Cvjetko Bubalo (mentor)
• Committee member: Kristina Radošević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2020-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Valorizacija otpadne kore naranĉe u sklopu koncepta biorafinerije primjer je odrţivog razvoja te predstavlja moguće rješenje za adekvatno zbrinjavanje agro-industrijskog otpada uz proizvodnju visokovrijednih proizvoda. Kako bi koncept biorafinerije u potpunosti bio u skladu s naĉelima zelene kemije, sve veći naglasak stavlja se na zamjenu konvencionalnih organskih otapala ''zelenijim'' alternativama poput primjene prirodnih eutektiĉkih otapala. U ovom radu istraţena je moguća primjena ĉetiri prirodna eutektiĉka otapala u valorizaciji naranĉine kore kao sirovine unutar koncepta biorafinerije. Ispitana otapala pokazala su se izrazito uspješnima prilikom ekstrakcije ukupnih polifenolnih spojeva, ali i kao pogodan medij za provoĊenje kinetiĉke rezolucije (R,S)-1-feniletil acetata pri ĉemu su pozitivno utjecala na enantioselektivnost biokatalizatora. S druge strane, ekstrakcija proteina i D-limonena iz naranĉine kore nije bila osobito uspješna u usporedbi s referentnim otapalima što ukazuje na potrebu za dodatnom optimizacijom odabranih otapala.

Abstract (english)

Valorization of orange peel waste through biorefinery concept is an excellent example of sustainable development. It represents a potential solution for environmentally friendly management of agro-industrial waste while also allowing production of high-value products. In order for biorefinery concept to be in accordance to green chemistry principles it is very important to introduce green solvents such as natural deep eutectic solvents (NaDES) to replace conventional organic solvents. Therefore, possible use of four NaDES in biorefinery of orange peel waste has been studied. NaDES proved to be very efficient in extraction of polyphenolic compounds, and also could be considered as a suitable medium for kinetic resolution of (R,S)-1-phenylethyl acetate showing a positive impact on the enantioselectivity of biocatalyst. However, extraction of proteins and D-limonene from orange peel did not meet the desired efficiency when compared to reference solvents suggesting that further optimization of used NaDES is needed.

• Keywords: kinetiĉka rezolucija; otpadna kora naranĉe; prirodna eutektiĉka otapala; proizvodi dodane vrijednosti; zelena kemija
• Keywords (english): green chemistry; kinetic resolution; natural deep eutectic solvents; orange peel waste; value-added products
• Language: croatian
• URN:NBN: urn:nbn:hr:159:281835
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-01-21 13:17:39