Pročišćavanje kravljeg kolostruma kontaminiranog antibioticima te izdvajanje imunoglobulina

master's thesis

• Title (english): Purification of bovine colostrum contaminated with antibiotics and separation of immunoglobulins
• Author: Marijela Bašić
• Mentor: Rajka Božanić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Milk and Milk Products); Zagreb
• Defense date and country: 2014-07-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Kolostrum je oblik mlijeka, proizveden u mliječnim žlijezdama u kasnoj trudnoći i nekoliko dana nakon poroda. Znanstvenici su se prvobitno zainteresirali za kolostrum zbog velikog udjela antitijela. Najčešće metode za preparativno pročišćavanje proteina uključuju precipitaciju i kromatografske metode. Postoje brojne metode za pročišćavanje imunoglobulina iz različitih medija, ali samo nekolicina se koristi za kravlji kolostrum. Za kontrolu kvalitete kolostruma je važna odsutnost antibiotika. Testovi za antibiotike koji se koriste u mljekarskoj industriji su napravljeni za mlijeko i ne postoje literaturni podaci koji ukazuju na mogućnost njihove primjene na kolostrumu. Rezultati tri česte metode za precipitaciju proteina (pomoću amonijevog sulfata, natrijevog sulfata, kaprilne kiseline) su pokazali da je najučinkovitija precipitacija sa 1.96% i 3.85% kaprilne kiseline koja rezultira koncentracijom kravljeg IgG od 80% od ukupnih proteina. Delvotest i Twinsensor, česti testovi na antibiotike u mljekarskoj industriji, su se pokazali učinkovitima na kravljem kolostrumu. Kationska ionska kromatografija se pokazala učinkovitom metodom za pročišćavanje imunoglobulina kravljeg kolostruma od antibiotika.

Abstract (english)

Colostrum is a form of milk produced by mammary glands in late pregnancy and few days after delivery. Scientists originally got interested in bovine colostrum because of its high antibody levels. The most common methods for preparative purification of proteins involve precipitation and chromatographic methods. There are numerous methods for purification of immunoglobulins from different media, but only few of them are used for bovine colostrum. For quality control of colostrum, the absence of antibiotics is important. Antibiotic tests used in dairy industry are designed for milk and there is no literature data about possibility of their usage on colostrum. Results of three frequent protein precipitation methods (by ammonium sulfate, sodium sulfate, caprylic acid) have showed that the most efficient is precipitation by 1.96% and 3.85% caprylic acid which results with concentration of 80% bovine IgG of total protein. Delvotest and Twinsensor, common antibiotic tests in dairy industry, have proven effective on bovine colostrum. Cation exchange chromatography has proved as effective method for purification of immunoglobulins in bovine colostrum of antibiotics.

• Keywords: kolostrum; imunoglobulini; pročišćavanje; antibiotici
• Keywords (english): colostrum; immunoglobulins; purification; antibiotics
• Language: english
• URN:NBN: urn:nbn:hr:159:710066
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 07:51:25