Racionalan dizajn niskotemperaturnih eutektičkih otapala za enantioselektivnu bioredukciju halogeniranog acetofenona

master's thesis

• Title (english): Rational design of deep eutectic solvents for enantioselective bioreduction of halogenated acetophenone
• Author: Ida Šimunčić
• Mentor: Marina Cvjetko Bubalo (mentor)
• Committee member: Ivana Radojčić Redovniković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2021-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Potreba za dizajniranjem kemijskih, farmaceutskih i biotehničkih proizvoda i procesa koji smanjuju ili potpuno uklanjaju primjenu i stvaranje škodljivih i opasnih tvari s jedinstvenim ciljem zaštite okoliša doveli su do razvoja koncepta zelene kemije koji je sve učestaliji industrijski pristup današnjice. Spomenuti pristup podrazumijeva korištenje alternativnih otapala i ekološki prihvatljivih i obnovljivih sirovina, ali i primjenu alternativnih oblika energije te provođenje organskih reakcija primjenom biokatalizatora. Shodno tome, sve je veći interes za primjenom eutektičkih otapala kao medija za biokemijsku pripravu kiralnih biološki aktivnih komponenti. U ovom je radu provedena reakcija enantioselektivne redukcije halogeniranog acetofenona u kiralni halogenirani alkohol, a spomenuta reakcija katalizirana je kvascem Saccharomyces cerevisiae, inače idealnim biokatalizatorom za industrijsku primjenu. Enantioselektivna redukcija provedena je u tri različita niskotemperaturna eutektička otapala s različitim udjelima vode. Biokatalitička reakcija redukcije u eutektičkom otapalu rezultirala je većim iskorištenjem u otapalu kolin klorid : etilen glikol s 10 %-tnim masenim udjelom vode odnosu na bioredukcije provedene u ostalim otapalima i puferu.

Abstract (english)

Designing non-polluting processes and the need to design chemical, pharmaceutical and biotechnical products and processes that reduce or completely eliminate the use and production of harmful and hazardous substances, with the unique goal of environmental protection, have led to the development of green chemistry, which is an increasingly common industrial approach today. This approach involves the use of alternative solvents and environmentally friendly and renewable raw materials, but also the use of alternative forms of energy and the implementation of organic reactions using biocatalysts. Consequently, there is a growing interest in the use of eutectic solvents as a medium for the biochemical preparation of chiral biologically active components. In this work, the reaction of enantioselective reduction of halogenated acetophenone to chiral halogenated alcohol was performed, and the mentioned reaction was catalyzed by the yeast Saccharomyces cerevisiae, otherwise and ideal biocatalysts for industrial application. Enantioselective reduction was performed in three different deep eutectic solvents with different water contents. The biocatalytic reduction in deep eutectic solvents choline chloride : ethylene glycol with 10 % of water resulted in a higher yield compared to the bioreduction perfomed in the other solvents and buffer.

• Keywords: biokataliza; halogenirani acetofenon; niskotemperaturna eutektička otapala; Saccharomyces cerevisiae; zelena kemija
• Keywords (english): biocatalysis; deep eutectic solvents; green chemistry; halogenated acetophenone; Saccharomyces cerevisiae
• Language: croatian
• URN:NBN: urn:nbn:hr:159:011786
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-18 10:57:55