Nefroprotektivni učinak polifenola iz cvijeta trnine na markere oksidacijskog stresa u miša

master's thesis

• Title (english): Nephroprotective effect polyphenols from trnina flower affect markers of oxidative stress in mouse
• Author: Lea Sabljić
• Mentor: Irena Landeka Jurčević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Chemistry and Biochemistry); Zagreb
• Defense date and country: 2016-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Djelovanje visoko-proteinske dijete (Whey protein) i polifenola izoliranih iz cvijeta trnine (TC) istraženo je na laboratorijskim životinjama (C57BL6 miš) kroz 30 dana tretmana. Životinje su bile podijeljene u 4 grupe: grupa I – kontrolna grupa; grupa II – TC (ekstrakt cvijeta trnine); grupa III – WP (Whey protein) i grupa IV – WP+TC. Istraživani biomarkeri oksidacijskog stresa u homogenatu tkiva bubrega bili su malondialdehid (MDA), karbonilirani proteini (PC), reducirani glutation (GSH), superoksid dismutaza (SOD) i katalaza (CAT). Rezultati pokazuju da je tretman s TC statistički značajno (ANOVA, p<0,05) smanjio količinu MDA i PC u bubregu miša u odnosu na kontrolnu skupinu životinja što se može pripisati polifenolima prisutnim u ekstraktu cvijeta trnine, dok su ostale grupe ostale na razini kontrolne skupine. Koncentracije GSH, SOD i CAT u bubregu statistički su značajno (ANOVA, p<0,05) povećane u svim grupama miševa u odnosu na kontrolnu grupu, što kazuje na antioksidacijski učinak TC kao i WP i smanjenje oksidacijskog stresa. Ovi rezultati ukazuju na to da ekstrakt cvijeta trnine i proteini sirutke mogu djelovati kao nutritivni sastojak za povećanje endogenih antioksidacijskih enzima i smanjenje oksidacijskog stresa.

Abstract (english)

The effect of high-protein diet (Whey protein) and polyphenols which are isolated from trnina flower (TC) has been investigated in laboratory animals (C57BL6 mouse). The animals were treated for 30 days and divided into 4 groups: group I - control group; Group II - TC (Flower Extract Trnine); Group III - WP (Whey protein) and Group IV - WP + TC. Biomarkers of oxidative stress which were studied in kidney tissue homogenates were malondialdehyde (MDA), carbonylated proteins (PC), reduced glutathione (GSH), superoksid dismutase (SOD) and catalase (CAT). The results show that TC statistically significant (ANOVA, p <0.05) reduced the activity of MDA and PC in the kidney of a mouse which were compared with control group of animals. That effect can be attributed with polyphenols present in the extract of trnina flowers. The other groups were unchanged and close to control group. The concentrations of GSH, SOD and CAT were statistically significant (ANOVA, p <0.05) increased in all groups of mouses compared with control group, which indicates the antioxidant effect of TC and WP and reducing oxidative stress. These results indicate that the extract of a trnina flower and whey proteins increase endogenous antioxidant enzymes and reduce oxidative stress.

• Keywords: Trnina (Prunus spinosa L.); oksidacijski stres; visoko-proteinska dijeta
• Keywords (english): Trnina (Prunus spinosa L.); oxidative stress; high protein diet
• Language: croatian
• URN:NBN: urn:nbn:hr:159:867961
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 08:54:36