Priprema i biološka aktivnost ekstrakata nusproizvoda vinske industrije pripravljenih pomoću niskotemperaturnih eutektičkih otapala

master's thesis

• Title (english): Preparation and biological activity of wine industry by-products extracts prepared using deep eutectic solvents
• Author: Iva Dvorneković
• Mentor: Ivana Radojčić Redovniković (mentor)
• Committee member: Kristina Radošević (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2021-11-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Za zelenu ekstrakciju biološki aktivnih spojeva posljednjih se godina često upotrebljavaju prirodna niskotemperaturna eutektička otapala (NADES), kao potencijalna zamjena konvencionalnim otapalima. Nakon proizvodnje vina zaostaju nusprodukti, pokožica i sjemenke grožđa, bogati polifenolima koji se mogu primijeniti u kozmetičkoj industriji. U ovom radu su pripremljeni ekstrakti pokožice i sjemenki grožđa u NADES-ima prethodno dizajniranima pomoću COSMOtherm programa i etanolu. Ukupan sadržaj polifenola u ekstraktima određen je Folin-Ciocalteu reagensom, određeni polifenoli identificirani su i kvatificirani tekućinskom kromatografijom visoke djelotvornosti, dok je antioksidacijski učinak ekstrakata određen ORAC metodom. Biološka aktivnost pripravljenih ekstrakata ispitana je in vitro na HaCaT staničnoj liniji MTS metodom, a antioksidacijski potencijal u stanicama DCFA-DA metodom. Najbolji učinak ekstrakcije postignut je eutektičkim otapalom betain : glukoza za pokožicu, a betain : etilen-glikolom za sjemenke. HPLC analizom određena je prisutnost epigalokatehina, katehina, epikatehina, rutin trihidrata i derivata flavan-3-ola u ekstraktima pokožice i sjemenka. NADES otapala, na temelju svih ispitanih parametara, mogu uspješno zamjeniti etanol kao konvencionalno otapalo u procesu ekstrakcije. S obzirom na uoče

Abstract (english)

In recent years, natural deep eutectic solvents (NADES) have often been used for extraction of biologically active compounds, as a potential replacement for conventional solvents. Grape skin and seeds are wine production by-products rich in polyphenols that can be used in cosmetic industry. In this study, grape skin and seeds extracts were prepared in NADES previously selected by COSMOtherm and ethanol. Extracts total polyphenols content was determined with Folin-Ciocalteu reagent and certain polyphenols were identified and quantified by high-performance liquid chromatography, while antioxidant effect by the ORAC method. Biological activity of prepared extracts was evaluated in vitro on the HaCaT Cell line using the MTS and antioxidant effect in cells using the DCFH-DA assays. Betaine: glucose provided the best extraction efficiency for grape skin, betaine : ethylene glycol for grape seeds. The presence of epigallocatechin, catechin, epicatechin, rutin trihydrate and derivatives flavan-3-ols in grape skin and seeds was also shown. Based on all tested parameters, NADES can successfully replace ethanol as conventional solvent in extraction processes. Given the observed proliferation of HaCaT cells, the tested extracts have the potential for application in the cosmetics industry.

• Keywords: prirodna niskotemperaturna eutektička otapala; polifenoli; sjemenke i pokožica grožđa; COSMOtherm; kozmetička industrija
• Keywords (english): natural deep eutectic solvents; polyphenols; grape skin and seeds; COSMOtherm; cosmetic industry
• Language: croatian
• URN:NBN: urn:nbn:hr:159:053280
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-12-07 14:34:59