Učinak proteinskih hidrolizata uljne pogače lana na rast HEK 293 stanične linije

undergraduate thesis

• Title (english): Effect of flaxseed cake protein hydrolysates on growth and metabolism of HEK 293 cell line
• Author: Laura Režek
• Mentor: Igor Slivac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2022-07-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U posljednje se vrijeme sve veća pažnja pridaje razvoju medija za uzgoj kultura životinjskih stanica koji ne sadrži serum, uslijed uočavanja njegovih brojnih nedostataka. Svoju primjenu u ovim tehnologijama pronašli su proteinski hidrolizati biljnog podrijetla koji su se pokazali kao učinkovit nu-tritivni dodatak. Kao izvor proteina dokazale su se tzv. uljne pogače sjemenki uljarica zaostale nakon postupaka ekstrakcije ulja. Radi se o industrijskom nusproizvodu relativno ograničene iskoristivosti. Cilj je ovog rada bio ispitati učinak proteinskih hidrolizata uljne pogače lana dobivenih pomoću enzima Alcalase, Neutrase i Protamex na rast i metabolizam HEK 293 stanične linije te mogućnost djelomične zamjene seruma u mediju za uzgoj stanica. Rezultati su pokazali da proteinski hidrolizati u koncentraciji od 0,5 g Lˉ¹ i 2,5 g Lˉ¹ mogu poslužiti kao zamjena dijela seruma kada se pripreme cijepanjem enzimom Neutrase. Hidrolizati pripremljeni enzimima Protamex i Alcalase pokazali su manju učinkovitost na rast stanica, pa čak i inhibitorno djelovanje. Manja koncentracija hidrolizata pokazala je bolji učinak na rast stanica nego hidrolizati u većoj koncentraciji.

Abstract (english)

Recently, more and more attention is paid to the development of media for the cultivation of animal cell cultures that do not contain the addition of serum, due to its many disadvantages. Protein hydrolysates of plant origin have found their application in these technologies and have proven to be an effective nutritional supplement. Oil cakes are side-products of oil extraction, an industrial residue rich in protein, have proven to be potential source of proteins to be digested into hydrolysates. The aim of this study was to examine the effect of flaxseed oil cake hydrolysates prepared by enzymes Alcalase, Neutrase and Protamex on the growth and metabolism of HEK 293 cell line, as well as the possibility of partial replacement of serum. The results showed that protein hydrolysates at a concentration of 0,5 g Lˉ¹ and 2,5 g Lˉ¹ can serve as a replacement for part of the serum when prepared by cleavage with the enzyme Neutrase. Hydrolysates prepared by the enzymes Protamex and Alcalase have shown lower efficacy on cell growth and even inhibitory activity. A lower concentration of hydrolysates showed a better effect on cell growth that hydrolysates of higher concentration.

• Keywords: proteinski hidrolizati; uljna pogača lana; HEK 293; kultura životinjskih stanica
• Keywords (english): protein hydrolysates; flaxseed oil cake; HEK 293; animal cell culture
• Language: croatian
• URN:NBN: urn:nbn:hr:159:207994
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-10-06 11:24:53