Utjecaj ultrazvuka visoke snage na prinos sekundarnih biljnih metabolita i proteina iz lista šećerne repe

master's thesis

• Title (english): Influence of high power ultrasound on yield of secondary plant metabolites and proteins from sugar beet leaves
• Author: Matea Hunić
• Mentor: Anet Režek Jambrak (mentor)
• Committee member: Sven Karlović (predsjednik povjerenstva)
• Committee member: Mojca Čakić Semenčić (član povjerenstva)
• Committee member: Filip Šupljika (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Management, Foreign Languages for Specific Purposes and Physical Education and Health); (Laboratory for Sustainable Development); Zagreb
• Defense date and country: 2022-07-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Primjenom ultrazvuka visoke snage (UAE) i toplinskog tretmana, ekstrahirani su ukupni proteini i fenoli iz sušenih i svježih listova šećerne repe uz vodu kao otapalo. Spektrofotometrijskom metodom utvrđen je veći prinos UAE u odnosu na toplinsku ekstrakciju. Najveći udio ukupnih proteina iznosi 147,9095 ± 4,58 mg (gs.tv.)ˉ¹, a fenola 17,8924 ± 0,38 mg (gs.tv.)ˉ¹, uz amplitudu 100 % i vrijeme tretiranja 9 min. Za UAE izračunate su vrijednosti promjene temperature, ukupne promjene energije i gustoće snage. Statističkom obradom u programu STATGRAPHICS, ispitan je utjecaj amplitude i vremena tretiranja na ukupne proteine i fenole, pH te električnu vodljivost; a utvrđen je kao statistički značajan (p<0,05) za ukupne proteine. Uz optimalnu vrijednost 100 %, amplituda kod svježih listova pokazuje p<0,05 za ukupne fenole, električnu vodljivost i ukupnu promjenu energije; a kod sušenih listova za pH. Utjecaj vremena tretiranja toplinske ekstrakcije na izlazne parametre, ispitan analizom varijance, statistički je značajan kod ukupnih proteina i fenola sušenih listova te ukupnih proteina i električne vodljivosti svježih listova.

Abstract (english)

Using high power ultrasound (UAE) and heat treatment, total proteins and phenolics were extracted from dried and fresh sugar beet leaves with water as solvent. Spectrophotometric method showed a higher UAE yield compared to thermal extraction. The highest value for total proteins was 147,9095 ± 4,58 mg (gDM)ˉ¹, and for total phenolics 17,8924 ± 0,38 mg (gDM)ˉ¹, achieved with an amplitude of 100 % and treatment time 9 min. For UAE, values of temperature change, total energy change and power density were calculated. Statistical analysis in the STATGRAPHICS program examined the effect of amplitude and treatment time on total proteins and phenolics, pH and electrical conductivity; and was found to be statistically significant (p<0,05) for total proteins. With an optimal value of 100 %, the amplitude in fresh leaves shows p<0,05 for total phenols, electrical conductivity, and total energy change; and in the case of dried leaves for pH. The influence of thermal extraction treatment time on the output parameters, examined by analysis of variance, is statistically significant for total proteins and phenols of dried leaves and total proteins and electrical conductivity of fresh leaves.

• Keywords: ekstrakcija potpomognuta ultrazvukom; toplinska ekstrakcija; proteini; fenoli; list šećerne repe
• Keywords (english): ultrasonic assisted extraction; heat extraction; proteins; phenolics; sugar beet leaf
• Language: croatian
• URN:NBN: urn:nbn:hr:159:342673
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-01-19 13:02:24