Određivanje biološkog potencijala frakcija morske makroalge Cystoseira compressa baziranog na pigmentima i njihovim derivatima

master's thesis

• Title (english): Determination of biological potential of Cystoseira compressa macroalgal fractions based on pigments and their derivatives
• Author: Karolina Begić
• Mentor: Ivona Elez Garofulić (mentor)
• Committee member: Verica Dragović-Uzelac (predsjednik povjerenstva)
• Committee member: Sanja Babić (član povjerenstva)
• Committee member: Maja Repajić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2022-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Morske makroalge predstavljaju bogat izvor strukturno različitih i biološki aktivnih molekula. Zbog uravnotežene nutritivne vrijednosti odlični su kandidati za implementaciju u prehrambenu industriju. Unutar ovog rada ispitan je biološki potencijal dviju nepolarnih (metanolna i diklormetanska) frakcija smeđe makroalge Cystoseira compressa. Antioksidativna aktivnost utvrđena je pomoću tri in vitro metode (ORAC, FRAP i DPPH) te potvrđena in vivo pomoću živog embrija zebrice Danio rerio. Inhibicija kolagenaze utvrđena je kod obje frakcije, s nešto većim postotkom zabilježenim kod metanolne frakcije. Diklormetanska frakcija potaknula je bržu regeneraciju amputirane repne peraje kod ličinaka zebrice Danio rerio, dok kod metanolne frakcije takav učinak nije zabilježen. Testom embriotoksičnosti na embrijima zebrice utvrđene su koncentracije testiranih frakcija bez toksičnog učinka. Usporedbom biološke aktivnosti s rezultatima kemijske analize, može se zaključiti kako su fukoksantin, feoforbid a i feofitini a i b, detektirani u obje ispitivane frakcije, jedni od glavnih spojeva odgovornih za dokazane pozitivne učinke. Dobiveni rezultati pokazuju kako makroalga Cystoseira compressa predstavljaja prirodan izvor bioaktivnih molekula, posebice pigmenata i njihovih derivata, koje se mogu primijeniti u prehrambenoj i kozmetičkoj industriji.

Abstract (english)

Marine macroalgae are a rich source of structurally diverse and biologically active molecules. Due to their balanced nutritional value, they are excellent candidates for implementation in the food industry. Within this work, the biological potential of two non-polar (methanolic and dichloromethane) fractions of brown macroalga Cystoseira compressa was examined. Antioxidant activity was determined using three in vitro methods (ORAC, FRAP, and DPPH) and confirmed in vivo using zebrafish Danio rerio embryos. Collagenase inhibition was confirmed on both fractions, with a slightly higher percentage recorded on the methanolic fraction. The dichloromethane fraction stimulated rapid regeneration of the amputated tail fin in Danio rerio zebrafish larvae, while no such effect was recorded on the methanolic fraction. The embryotoxicity test on zebrafish embryos determined the concentration of tested fractions with no toxic effects. By comparing the biological activity with the results of the chemical analysis, it can be concluded that fucoxanthin, pheophorbid a and pheophytins a and b, detected in both tested fractions, are one of the main compounds responsible for the proven beneficial effects. The obtained results show that Cystoseira compressa represents a natural source of bioactive molecules, especially pigments and their derivates, that can be used in the food and cosmetic industry.

• Keywords: pigmenti; smeđe makroalge; antioksidativni potencijal; kolagenaza; modelni organizam
• Keywords (english): pigments; brown macroalgae; antioxidant potential; collagenase; model organism
• Language: croatian
• URN:NBN: urn:nbn:hr:159:232645
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-03-15 11:35:46