Utjecaj inkapsulacije na funkcionalnost i stabilnost fenolnih spojeva iz ekstrakta cvijeta trnine (Prunus spinosa flos) tijekom sušenja raspršivanjem

doctoral thesis

• Title (english): Influence of encapsulation on the functionality and stability of phenolic compounds from blackthorn flower (Prunus spinosa flos) extract during spray drying
• Author: Nikolina Gaćina
• Mentor: Ivona Elez Garofulić (mentor)
• Committee member: Verica Dragović-Uzelac (predsjednik povjerenstva)
• Committee member: Stela Jokić (član povjerenstva)
• Committee member: Martina Bituh (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2023-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Cilj ovog rada bio je optimizirati uvjete inkapsulacije fenolnih spojeva cvijeta trnine (Prunus spinosa flos) primjenom postupka sušenja raspršivanjem. Korišteni nosači su maltodekstrin (MD 13-17 DE), smjesa maltodekstrina i 25% arapske gume (MD+25%GA), smjesa maltodekstrina i 50% arapske gume (MD+50%GA) te smjesa maltodekstrina i 25% inulina (MD+25%IN) i smjesa maltodekstrina i 50% inulina (MD+50%IN). Ispitivan je i utjecaj omjera nosača i suhe tvari ekstrakta (0,5; 1 i 2) te temperature inkapsulacije (120, 150 i 180°C) na stabilnost fenolnih spojeva i njihov antioksidacijski kapacitet. Ovim istraživanjem obuhvaćeno je određivanje ukupnih hidroksicimetnih i hidroksibenzojevih kiselina, flavonola, flavanola i flavona, te ukupnih fenolnih spojeva primjenom ultradjelotvorne tekućinske kromatografije (UPLC) uz MS/MS detekciju, dok je antioksidacijski kapacitet određen ORAC metodom. Vrsta nosača je određena kao ključan faktor zadržavanja svih skupina spojeva u inkapsuliranim ekstraktima cvijeta trnine dok temperatura i omjer nosača i suhe tvari ekstrakta nisu imali značajan utjecaj. Optimalni uvjeti inkapsulacije su: nosač MD, omjer nosača i suhe tvari ekstrakta 1:1 te temperatura sušenja 180°C. Pri navedenim optimalnim uvjetima zadržavanje ukupnih fenolnih spojeva je iznosilo 91,24% i AOK 81,30% u odnosu na početni ekstrakt. Stoga inkapsulirani ekstrakti cvijeta trnine dobiveni pri navedenim optimalnim uvjetima imaju visok potencijal za razvoj funkcionalnih proizvoda s poboljšanom stabilnošću fenolnih spojeva i visokom antioksidativnom aktivnošću.

Abstract (english)

This work aimed to optimize the conditions of encapsulation of phenolic compounds of blackthorn flower (Prunus spinosa flos) using spray drying method. Wall materials used are maltodextrin (MD 13-17 DE), a mixture of maltodextrin and 25% gum arabic (MD+25%GA), a mixture of maltodextrin and 50% gum arabic (MD+50%GA) and a mixture of maltodextrin and 25% inulin (MD +25%IN) and a mixture of maltodextrin and 50% inulin (MD+50%IN). The influence of wall materail to extract dry matter ratio (0.5; 1 and 2) and the encapsulation temperature (120, 150 and 180°C) on the stability of phenolic compounds and their antioxidant capacity was also investigated. This study included the determination of total hydroxycinnamic and hydroxybenzoic acids, flavonols, flavanols and flavones, and total phenolic compounds using ultra-efficient liquid chromatography (UPLC) with MS/MS detection, while antioxidant capacity was determined by ORAC method. The type of wall material was determined as a key factor in the retention of all phenolic groups in encapsulated blackthorn flower extracts, while the temperature and wall material to extract dry matter ratio had no significant effect. The optimal encapsulation conditions are: MD as wall materail, wall materail to extract dry matter ratio of 1:1 and drying temperature of 180°C. Under the specified optimal conditions, retention of total phenolic compounds was 91.24% and AOK 81.30% compared to the initial extract. Therefore, the encapsulated blackthorn flower extracts obtained under the specified optimal conditions have a high potential for the development of functional products with improved stability of phenolic compounds and high antioxidant activity.

• Keywords: inkapsulacija; fenolni spojevi; sušenje raspršivanjem; cvijet trnine; ORAC
• Keywords (english): encapsulation; polyphenols; spray drying; blackthorn flower; ORAC
• Language: croatian
• URN:NBN: urn:nbn:hr:159:589845
• Promotion: 2023
• Study programme: Title: Postgraduate PhD Study in Nutrition; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam (doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam)
• Type of resource: Text
• Extent: 181 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-05-03 13:18:27