Primjena blisko infracrvene spektroskopije u detekciji pesticida prisutnih u jabuci

master's thesis

• Title (english): Application of near infrared spectroscopy in detection of pesticides present in apples
• Author: Andrea Klapan Kurtov
• Mentor: Tamara Jurina (mentor)
• Committee member: Jasenka Gajdoš Kljusurić (predsjednik povjerenstva)
• Committee member: Maja Benkovic (član povjerenstva)
• Committee member: Danijela Bursać Kovačević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Process Engineering); (Laboratory for Measurement, Regulation and Automatisation); Zagreb
• Defense date and country: 2022-07-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Cilj ovog rada bio je istražiti može li se primjenom NIR spektroskopije (engl. Near Infrared Spectroscopy, NIR) kvalitativno odrediti/detektirati prisutnost fungicida (Chromosul 80 i Cuprablau Z 35 WG) na površini jabuka. Za svaki fungicid je pripremljeno osam jabuka od kojih su dvije činile kontrolu, a ostale su prskane originalnim suspenzijama/otopinama istraživanih fungicida i njihovim razrjeđenjima od sto i tisuću puta. Jabuke su snimane pomoću NIR instrumenta tijekom tri tjedna. Kvalitativno detektiranje fungicida interpretacijom izvornih NIR spektara pokazalo se slabo informativnim u pronalaženju razlika među njima. Kombinacijom snimljenih NIR spektara i primjenom PCA (engl. Principal Component Analysis, PCA) analize uspješno su razdvojeni originalni uzorci suspenzija/otopina fungicida od njihovih odgovarajućih razrjeđenja te uzorci jabuka na temelju razlike u koncentraciji fungicida kojima su prskane. Kako bi se ustanovila povezanost između NIR spektara i fizikalnih karakteristika (ukupne otopljene tvari, vodljivost, boja) odgovarajućih suspenzija/otopina fungicida i njihovih odgovarajućih razrjeđenja, razvijeni su PLS (engl. Partial Least Squares, PLS) modeli koji su pokazali da postoji linearna povezanost između NIR spektara te fizikalnih karakteristika (ukupne otopljene tvari, vodljivost) istraživanih fungicida (R2 ≥ 0,9).

Abstract (english)

The aim of this work was to investigate whether the presence of fungicides (Chromosul 80 and Cuprablau Z 35 WG) on the apple surface can be qualitatively determined/detected using NIR (Near Infrafred) spectroscopy. Eight apples were prepared for each fungicide, two of them presented control, and the rest were sprayed with the original suspensions/solutions of the investigated fungicides and their dilutions of 100 and 1000 times. Apples were recorded using the NIR instrument during a period of three weeks. Qualitative detection of the fungicides by interpretation of their original NIR spectra was poorly informative in finding differences between them. The combination of the NIR spectra and the application of PCA (Principal Component Analysis) successfully separated the original samples of suspensions/solutions of the fungicides from their corresponding dilutions and sprayed apple samples based on the difference in the concentration of the fungicides. In order to establish the relationship between the NIR spectra and the physical characteristics (total dissolved solids, conductivity, color) of the fungicide suspensions/solutions, PLS (Partial Least Squares) models were developed and showed linear relationship between the NIR spectra and the physical characteristics (total dissolved solids, conductivity) of the investigated fungicides (R2 ≥ 0.9).

• Keywords: NIR spektroskopija; kemometrija; ostaci pesticida; jabuka; PLS modeli
• Keywords (english): NIR spectroscopy; chemometrics; pesticide residues; apples; PLS models
• Language: croatian
• URN:NBN: urn:nbn:hr:159:916408
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-10-06 10:00:24