Osnove metabolomike temeljene na spektrometriji masa i njezina primjena

undergraduate thesis

• Title (english): Mass spectrometry-based metabolomics: fundamentals and its application
• Author: Lovro Bilić
• Mentor: Anita Horvatić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Physical Chemistry and Corrosion); Zagreb
• Defense date and country: 2023-07-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Metabolomika, definirana kao sveobuhvatna analiza malih molekula ili metabolita u biološkom uzorku, tehnologija je u nastajanju koja omogućuje karakterizaciju metaboličkih fenotipa. Različita fizikalna svojstva metabolita zahtijevaju kompleksne analitičke pristupe kao i primjenu različitih analitičkih platformi poput spektrometrije masa, kromatografije i nuklearne magnetske rezonancije ne bi li se nedvojbeno identificirali i kvantificirali metaboliti. U ovom radu opisane su osnove metabolomike, kao i suvremene analitičke tehnike temeljeni na spektrometriji masa koji se primjenjuju u analizi humanog metaboloma dajući uvid u metaboličke promjene povezane s bolestima te prehranom. Nadalje, naglašeno je značenje metabolomike kao alata za otkrivanje novih lijekova.

Abstract (english)

Metabolomics, defined as a comprehensive analysis of small molecules or metabolites in a biological sample, is an emerging technology that enables the characterization of metabolic phenotypes. The different physical properties of metabolites require complex analytical strategies and the application of various analytical platforms such as mass spectrometry, chromatographic techniques, and nuclear magnetic resonance to unambiguously identify and quantify metabolites. This thesis describes the basics of metabolomics, as well as modern mass spectrometry-based analytical approaches, used in the analysis of the human metabolome, providing insight into metabolic changes associated with diseases, nutrition, as well as the discovery of new drugs.

• Keywords: metabolomika; spektrometrija masa; nutricionizam; medicina; farmaceutika
• Keywords (english): metabolomics; mass spectrometry; medicine; nutrition; pharmaceutical industry
• Language: croatian
• URN:NBN: urn:nbn:hr:159:699393
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-24 12:11:24