Antimikrobna aktivnost ekstrakata cvijeta industrijske konoplje

undergraduate thesis

• Title (english): Antimicrobial activity of industrial hemp inflorescence extracts
• Author: Klara Rončević
• Mentor: Karla Hanousek Čiča (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2023-09-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Istraživanja industrijske konoplje, Cannabis sativa, navode sposobnost antimikrobnog djelovanja ekstrakata ove biljke posebice na gram-pozitivne bakterije, uključujući multirezistentne patogene. U ovome je radu uspoređena antimikrobna aktivnost triju ekstrakata cvijeta industrijske konoplje koji su dobiveni različitim metodama ekstrakcije: klasičnom, mikrovalnom i ultrazvučnom. Ekstrakti su pripremljeni u karboksiliranom i dekarboksiliranom obliku u koncentracijama do 10 mg/mL. Ispitivanje je provedeno na gram-pozitivnim i gram-negativnim bakterijama, bakterijama mliječne kiseline i kvascima. Antimikrobna aktivnost određena je primjenom standardne disk difuzijske metode, dok je minimalna inhibitorna koncentracija (MIK) određena pomoću resazurin boje. Osjetljivima su se pokazale jedino gram-pozitivne bakterije s većom osjetljivošću na karboksilirane uzorke u usporedbi s dekarboksiliranima. Najniže MIK, 0,0156 mg/mL za Staphylococcus aureus i 0,25 mg/mL za Bacillus subtilis, zabilježene su za ekstrakte dobivene ultrazvučnom ekstrakcijom. Suprotno tome, gram-negativne bakterije (Escherichia coli, Pseudomonas aeruginosa i Salmonella typhimurium) te bakterije mliječne kiseline i kvasci nisu pokazali osjetljivost na testirane ekstrakte.

Abstract (english)

Studies of industrial hemp (Cannabis sativa) have confirmed antimicrobial efficacy of its’ extracts, especially on Gram-positive bacteria, including multidrug-resistant pathogens. This study was conducted to compare antimicrobial activity of three different samples obtained from the same hemp inflorescence extracted using traditional, microwave and ultrasound assisted extraction. Extracts were used in carboxylated and decarboxylated form in concentrations up to 10 mg/mL. Antimicrobial activity was tested on gram-positive and gram-negative bacteria, lactic acid bacteria and yeast. The antimicrobial effect was evaluated using the standard disc-diffusion method, while minimum inhibitory concentration (MIC) was determined using resazurin colorimetry. Only Gram-positive bacteria have shown sensitivity to all extracts, with a higher sensitivity to carboxylated extracts compared to the decarboxylated extracts. Lowest MIC, 0.0156 mg/mL for Staphylococcus aureus and 0.25 mg/mL for Bacillus subtilis was exhibited by the ultrasound assisted extracts. Conversely, no discernible indications of inhibition were observed within Gram-negative bacteria (E. coli, P. aeruginosa and S. typhimurium), lactic acid bacteria and yeast.

• Keywords: antimikrobna aktivnost; kanabinoidi; industrijska konoplja; ekstrakcija
• Keywords (english): antimicrobial activity; cannabinoids; industrial hemp; extraction
• Language: croatian
• URN:NBN: urn:nbn:hr:159:834179
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-29 08:33:32