Enzyme activity screening of different Ceratocystis paradoxa strains

master's thesis

• Title (croatian): Određivanje enzimske aktivnosti različitih sojeva plijesni Ceratocystis paradoxa
• Author: Magdalena Barišić
• Mentor: Antonija Trontel (mentor)
• Committee member: Mario Novak (predsjednik povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Committee member: Jasna Novak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2023-05-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

In this work, qualitative screening of amylolytic, lignocellulolytic and proteolytic enzyme activity was performed for four strains of filamentous fungus Ceratocystis paradoxa (strains DSM 63054, CBS 101054, CBS 374.83, CBS 453.66). Screening was performed using colorimetric agar plates filled with 24 different commercial-coloured polymers. After preliminary tests, cultivations of C. paradoxa strains on different media was performed to determine if these strains have considerable cellulase, xylanase, galactanase and/or amylolytic activity. Media used in these cultivations were standard yeast extract peptone dextrose medium (YPD), and media containing starch (i.e., potato dextrose medium and medium containing waste bread) or lignocellulosic raw materials (i.e., wheat bran). Assays were performed in culture supernatants spectrophotometrically using colored substrates (red starch, azo-cmcellulose, azo-xylan, azo-galactan). Strain DSM 63054 had highest cellulase activity (1150 mU mL⁻¹ ) in medium containing wheat bran and xylanase activity (1400 mU mL⁻¹ ) determined in medium containing waste bread, amongst all tested strains. Highest galactanase activity was determined for strain CBS 101054 in medium containing wheat bran (2100 mU mL⁻¹ ), while highest amylolytic activity was determined for the same strain in medium containing waste bread (2239 mU mL⁻¹ ).

Abstract (croatian)

U ovom radu provedeno je kvalitativno određivanje amilolitčke, celulolitičke i proteolitičke aktivnosti za četiri soja plijesni Ceratocystis paradoxa (DSM 63054, CBS 101054, CBS 374.83, CBS 453.66). Određivanje enzimske aktivnosti provedeno je korištenjem agar podloga u koijima se nalazi 24 različita komercijalna obojana polimera. Nakon preliminarnih testova, sojevi plijesni C. paradoxa uzgojeni su u različitim podlogama kako bi se utvrdilo imaju li ovi sojevi značajnu celulaznu, ksilanaznu, galaktanaznu i/ili amilaznu aktivnost. Podloge korištene za uzgoj ovih plijesni su podloga koja se sastoji od kvaščevog ekstrakta, peptona i glukoze (YPD) i podloge koje sadrže škrob (tj. podloga s krumpirom i glukozom te podloga s otpadnim kruhom) ili lignocelulozne sirovine (pšenične mekinje). Određivanje enzimske aktivnosti provedeno je u supernatantima spektrofotometrijski s različitim supstratima (crveni škrob, azo-cm-celuloza, azo-ksilan i azo-galaktan). Najviša celulazna (1150 mU mL⁻¹ ) aktivnost u podlozi sa pšeničnim mekinjama te ksilanazna (1400 mU mL⁻¹ ) u podlozi sa otpadnim kruhom, određena je za soj DSM 63054, u usporedbi s drugim testiranim sojevima. Najviša galaktanazna aktivnost određena je za soj CBS 101054 u podlozi s pšeničnim mekinjama (2100 mU mL⁻¹ ), te je za isti soj određena i maksimalna amilazna aktivnost u podlozi s otpadnim kruhom (2239 mU mL⁻¹).

• Keywords: ceratocystis paradoxa; amylase; xylanase; cellulase; galactanase
• Keywords (croatian): ceratocystis paradoxa; amilaze; ksilanaze; celulaze; galaktanaze
• Language: english
• URN:NBN: urn:nbn:hr:159:418978
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-15 08:05:33