Validacija metode za određivanje tokoferola u ulju pasjeg trna

master's thesis

• Title (english): Validation of method for the determination of tocopherols in sea buckthorn oil
• Author: Nikolina Knezović
• Mentor: Zoran Zorić (mentor)
• Committee member: Marko Obranović (predsjednik povjerenstva)
• Committee member: Maja Repajić (član povjerenstva)
• Committee member: Sandra Pedisić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for drying Technologies and monitoring of biologically active compounds); Zagreb
• Defense date and country: 2023-04-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Pasji trn (Hippophae rhamnoides L.) listopadna je grmolika biljka koja sadrži plod narančasto-crvene boje. Od ploda pasjeg trna proizvodi se ulje koje sadrži brojne bioaktivne komponente, kao što su karotenoidi i tokoferoli, koji su izrazito značajni s obzirom da pokazuju pozitivan utjecaj na zdravlje. Cilj ove preliminarne studije bio je validacija HPLC metode za određivanje tokoferola u ulju pasjeg trna, pri čemu su ispitani sljedeći parametri: selektivnost, točnost, preciznost, linearnost te granice detekcije i kvantifikacije. Dobivene vrijednosti za preciznost i točnost te granice detekcije i kvantifikacije pokazale su prikladnost razvijene HPLC metode za određivanje tokoferola. HPLC metodom u ulju pasjeg trna detektiran je jedino α-tokoferol. Rezultati statističke analize pokazali su da ulje sorte Leikora sadrži viši udio α-tokoferola, dok ulje sorte Ascola pokazuje veću antioksidacijsku aktivnost. Ekstrakcija superkritičnim CO₂ pokazala je bolje rezultate u odnosu na Soxhlet ekstrakciju.

Abstract (english)

Sea buckthorn (Hippophae rhamnoides L.) is a deciduous bushy plant with an orange-red fruit. Sea buckthorn oil contains numerous bioactive components, like carotenoids and tocopherols, which are extremely important because they show a positive impact on health. The aim of this preliminary study was to validate the HPLC method for the determination of tocopherols in sea buckthorn oil, where the following parameters were tested: selectivity, accuracy, precision, linearity and limits of detection and quantification. The obtained values for precision and accuracy, as well as detection and quantification limits showed the suitability of the developed HPLC method for the determination of tocopherols. Only α-tocopherol was detected in sea buckthorn oil by the HPLC method. The results of the statistical analysis showed that the oil of the Leikora variety contains a higher content of α-tocopherol, while the oil of the Ascola variety shows a higher antioxidant activity. Supercritical CO₂ extraction showed better results when compared to Soxhlet extraction.

• Keywords: validacija; visoko učinkovita tekućinska kromatografija; tokoferoli; ulje pasjeg trna; antioksidacijski kapacitet
• Keywords (english): validation; high performance liquid chromatography; tocopherols; sea buckthorn oil; antioxidant capacity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:339747
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-16 12:53:08