Izrada sustava za generiranje izvještaja analiza humanog crijevnog mikrobioma

master's thesis

• Title (english): Development of a system for generation of human intestinal microbiome analysis reports
• Author: Selma Šerbečić
• Mentor: Janko Diminić (mentor)
• Committee member: Jurica Žučko (predsjednik povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Committee member: Jasna Novak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2023-02-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Posljednjih godina crijevnoj mikrobioti čovjeka posvećuje se sve veća pozornost budući da mikroorganizmi koji nastanjuju gastrointestinalni trakt imaju važnu ulogu u bolesti i zdravlju domaćina. Kod zdravog pojedinca sastav crijevne mikrobiote relativno je stabilan, ali je podložan različitim čimbenicima koji mogu dovesti do disbioze crijeva što za posljedicu može imati razvoj određenih bolesti. U ovom radu nakon sekvenciranja umnoženih gena podaci su obrađeni u QIIME 2 programu te Microsoft Excelu nakon čega se koristio generirani automatizirani Word kako bi svaki ispitanik dobio izvještaj o sastavu crijevne mikrobiote. Analizom dobivenih rezultata istraživane populacije ustanovljeno je da su Firmicutes i Bacteroidetes dva najdominantnija koljena, a Bacteroides najzastupljeniji bakterijski rod koji je detektiran kod svih ispitanika. S druge strane, rod Prevotella detektiran je kod samo 4 ispitanika.

Abstract (english)

In recent years, the human intestinal microbiota has received increasing attention, since the microorganisms that inhabit the gastrointestinal tract play an important role in diseases and health of the host. In a healthy individual, the composition of the intestinal microbiota is relatively stable, but it is liable to various factors that can lead to intestinal dysbiosis, which can result in the development of certain diseases. In this work, after the amplified genes were sequenced, the data were processed in the QIIME 2 program and Microsoft Excel, after which the generated automated Word was used to give each subject a report on the composition of the intestinal microbiota. Analyzing the results of the researched population, it was established that Firmicutes and Bacteroidetes are the two most dominant phylum, and Bacteroides is the most represented bacterial genus which was detected in all subjects. On the other hand, the genus Prevotella was detected in only 4 subjects.

• Keywords: crijevna mikrobiota; QIIME program; Microsoft Excel; Microsoft Word
• Keywords (english): gut microbiota; QIIME pipeline; Microsoft Excel; Microsoft Word
• Language: croatian
• URN:NBN: urn:nbn:hr:159:151058
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-17 11:32:54