Robiotički i postbiotički potencijal kvasca Kluyveromyces marxianus DS12

master's thesis

• Title (english): Probiotic and postbiotic potential of the yeast Kluyveromyces marxianus DS12
• Author: Valerija Agičić
• Mentor: Jadranka Frece (mentor)
• Mentor: Deni Kostelac (pomoć pri izradi, neposredni voditelj)
• Committee member: Ksenija Markov (predsjednik povjerenstva)
• Committee member: Jasna Mrvčić (član povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2023-11-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U nastojanju da se proširi spektar istraženih i dokumentiranih probiotičkih sojeva, istraživanja probiotičkog potencijala u poboljšanju zdravlja crijevne mikrobiote i jačanju imunosnog sustava temelj su boljeg razumijevanja probiotičkog koncepta i razvoja novih probiotičkih proizvoda. Ovaj diplomski rad istražuje probiotički i postbiotički potencijal soja Kluyveromyces marxianus DS12, evaluirajući niz funkcionalnih parametara važnih za njegovu primjenu u probiotičkim formulacijama. Antimikrobna svojstva soja ispitana su protiv širokog spektra patogena, a analizirana je i sposobnost formiranja biofilmova, ključna za preživljavanje i kolonizaciju u crijevnim uvjetima. Autoagregacijske i koagregacijske karakteristike ocijenjene su kako bi se utvrdila interakcija soja s crijevnom mikrobiotom, a antioksidacijski potencijal ispitivan je kroz sposobnost neutralizacije DPPH slobodnih radikala. Utvrđena je i hemolitička aktivnost te reakcija na oksidacijski stres, kao i sposobnost soja da održi vitalnost u simuliranim uvjetima gastrointestinalnog trakta. Rezultati ovih sveobuhvatnih analiza pružaju temelj za daljnju primjenu soja K. marxianus DS12 kao probiotičkog agensa u funkcionalnoj hrani i terapiji. Rezultati provedenih eksperimenata potvrđuju da soj Kluyveromyces marxianus DS12 posjeduje obećavajuće karakteristike potrebne za kvalitetnog probiotičkog kandidata, a uz daljnje temeljite analize, ističe se i potencijal za postbiotičke aplikacije.

Abstract (english)

In the effort to expand the spectrum of probiotic strains, the investigation of probiotic potential in enhancing gut microbiota health and strengthening the immune system is foundational for a better understanding of the probiotic concept and the development of new probiotic products. This thesis investigates the probiotic and postbiotic potential of the Kluyveromyces marxianus DS12 strain, evaluating a range of functional parameters important for its application in probiotic formulations. The antimicrobial properties of the strain were tested against a broad spectrum of pathogens, and its ability to form biofilms, crucial for survival and colonization in the gut conditions, was analyzed. Auto-aggregation and co-aggregation characteristics were assessed to determine the strain's interaction with the gut microbiota, and antioxidant potential was examined through the ability to neutralize DPPH free radicals. Hemolytic activity and response to oxidative stress were also determined, as well as the strain's ability to maintain viability in simulated gastrointestinal conditions. The results of these comprehensive analyses provide a basis for the further application of the K. marxianus DS12 strain as a probiotic agent in functional foods and therapy. The outcomes of the conducted experiments confirm that the Kluyveromyces marxianus DS12 strain possesses promising characteristics needed for a quality probiotic candidate, and with further in-depth analyses, its potential for postbiotic applications is also highlighted.

• Keywords: probiotici; postbiotici; kvasci; Kluyveromyces marxianus; mikrobiota
• Keywords (english): probiotics; postbiotics; yeasts; Kluyveromyces marxianus; microbiota
• Language: croatian
• URN:NBN: urn:nbn:hr:159:512702
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-04 13:31:39