Potencijal niskotemperaturnih eutektičkih otapala za zamjenu dimetilsulfoksida

master's thesis

• Title (english): Potential of deep eutectic solvents to replace dimethyl sulfoxide
• Author: Sunčica Šarac
• Mentor: Kristina Radošević (mentor)
• Mentor: Martina Bagović (pomoć pri izradi, neposredni voditelj)
• Committee member: Ana Bielen (predsjednik povjerenstva)
• Committee member: Marina Cvjetko Bubalo (član povjerenstva)
• Committee member: Teuta Murati (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2023-12-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Zbog rastućeg broja dokaza o toksičnom djelovanju dimetilsulfoksida (DMSO) na ljudsko zdravlje potrebno je pronaći alternativna otapala koja će moći zamijeniti široko korišteni DMSO. Niskotemperaturna eutektička otapala (engl. Deep Eutectic Solvents, DES) su niskotoksična i biorazgradiva te se jednostavno pripravljaju. U ovom radu ispitana je citotoksičnost četiri DES-a (Bet:U, TMAO:Gua, TMAO:U i GPC:U) prema HeLa i HaCaT stanicama te je najslabije citotoksično djelovanje, slabije od DMSO-a, pokazao Bet:U. Odabrani DES-ovi (Bet:U, TMAO:U i ChCl:U) također su primijenjeni kao krioprotektivni agensi pri zamrzavanju HeLa i HaCaT staničnih linija, ali niti jedno niskotemperaturno eutektičko otapalo nije iskazalo krioprotektivna svojstva jednaka ili bolja DMSO-u. Primjenom COSMOtherm programa predviđena je topljivost ferocena u hidrofilnim i hidrofobnim DESovima te je pokazano da su hidrofobni DES-ovi bolji izbor za otapanje ferocena. Također je ispitana mogućnost otapanja biokonjugata ferocena i derivata 2-tiouracila u Ty:C₈ i Me:C₈ te potom učinak otopina spojeva u DES-ovima na HeLa staničnoj liniji. Pri ispitanim koncentracijama nije zapažen značajniji učinak biokonjugata ferocena i derivata 2-tiouracila na proliferaciju stanica, u odnosu na kontrolne stanice.

Abstract (english)

Increasing evidence of the toxic effects of dimethyl sulfoxide (DMSO) on human health made it necessary to find alternative solvents that can replace the widely used DMSO. Deep eutectic solvents (DES) are low-toxic, biodegradable and easily prepared. In this study, the cytotoxicity of four DESs (Bet:U, TMAO:Gua, TMAO:U, and GPC:U) towards HeLa and HaCaT cells was investigated, with Bet:U showing the least cytotoxic effect, which was lower than that of DMSO. Selected DESs (Bet:U, TMAO:U, and ChCl:U) were also applied as cryoprotective agents for HeLa and HaCaT cell lines, but none of the deep eutectic solvents showed cryoprotective properties equal to or better than DMSO. By using the COSMOtherm program, the solubility of ferrocene in hydrophilic and hydrophobic DES has been predicted, demonstrating that hydrophobic DESs are a better choice for dissolving ferrocene. Additionally, the possibility of dissolving bioconjugates of ferrocene and 2-thiouracil derivative in Ty:C₈ and Me:C₈ was examined, followed by assessing the effect of solutions on HeLa cell line. At the tested concentrations, there was no significant impact of bioconjugates of ferrocene and 2-thiouracil derivative on cell proliferation compared to control cells.

• Keywords: niskotemperaturna eutektička otapala; dimetilsulfoksid; biokonjugati ferocena i derivata 2-tiouracila; krioprezervacija; citotoksičnost
• Keywords (english): deep eutectic solvents; dimethyl sulfoxide; bioconjugates of ferrocene and 2-thiouracil derivative; cyopreservation; cytotoxicity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:946689
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-05 08:04:59