Razvoj LC-MS/MS metode za određivanje glukozinolata u listu raštike (Brassica oleracea var. acephala)

master's thesis

• Title (english): Development of LC-MS/MS method for determination of glucosinolates in kale leaf (Brassica oleracea var. acephala)
• Author: Iva Marinović
• Mentor: Marina Krpan (mentor)
• Mentor: Nikola Major (komentor)
• Mentor: Tvrtko Karlo Kovačević (pomoć pri izradi, neposredni voditelj)
• Committee member: Mirjana Hruškar (predsjednik povjerenstva)
• Committee member: Nikola Major (član povjerenstva)
• Committee member: Ines Panjkota-Krbavčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Quality Control); Zagreb
• Defense date and country: 2023-07-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Glukozinolati su sekundarni biljni metaboliti koji sadrže sumpor, a u najvećoj su količini zastupljeni u vrstama iz porodice kupusnjača. Cilj ovoga rada bio je razviti metodu tekućinske kromatografije visoke djelotvornosti u sprezi sa spektrometrijom masa (LC-MS/MS) za određivanje pojedinih glukozinolata te primijeniti istu na analizu glukozinolata u listu raštike (Brassica oleracea var. acephala). Identificirano je i kvantificirano 14 glukozinolata; osam alifatskih, tri indolna i tri aromatska glukozinolata u šest različitih primki raštike. Prosječna koncentracija ukupnih glukozinolata kretala se između 8741 mg/kg suhe tvari do 4771 mg/kg suhe tvari. Najzastupljeniji glukozinolati bili su glukoiberin, glukobrasicin i sinigrin, dok su glukozinolati s najnižim zabilježenim koncentracijama bili glukotropeolin, epiprogoitrin i glukolimnantin.

Abstract (english)

Glucosinolates are sulfur-containing plant secondary metabolites that are mostly present in Brassicaceae. The aim of this study was to develop a method of liquid chromatography with mass spectrometry (LC-MS/MS) for determination of glucosinolates and apply it to the determination of glucosinolates in kale leaf (Brassica oleracea var. acephala). A total of 14 glucosinolates in six different kale accessions were quantified in this study, including eight aliphatic glucosinolates, three indolic glucosinolates and three aromatic glucosinolates. The average concentration of total glucosinolates ranged from 8741 mg/kg dry matter to 4771 mg/kg dry matter. The most abundant glucosinolates were glucoiberin, glucobrassicin and sinigrin, while glucosinolates with the lowest detected concentrations were glucotropaeolin, epiprogoitrin and glucolimnanthin.

• Keywords: LC-MS/MS; ciljane analize; ionizacija elektroraspršenjem; glukozinolati; Brassica oleracea var. Acephala
• Keywords (english): LC-MS/MS; targeted analysis; electrospray ionization; glucosinolates; Brassica oleracea var. acephala
• Language: croatian
• URN:NBN: urn:nbn:hr:159:029987
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-07 07:11:57