Optimiranje ekstrakcije polifenola iz komine grožđa potpomognute ultrazvukom u niskotemperaturnim eutektičkim otapalima

master's thesis

• Title (english): Optimization of ultrasound-assisted extraction of polyphenols from grape pomace in deep eutectic solvents
• Author: Anamarija Mlinac
• Mentor: Ivana Radojčić Redovniković (mentor)
• Mentor: Manuela Panić (pomoć pri izradi, neposredni voditelj)
• Committee member: Filip Dujmić (predsjednik povjerenstva)
• Committee member: Kristina Radošević (član povjerenstva)
• Committee member: Mladen Brnčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2023-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Posljednjih desetljeća sve se veća pažnja usmjerava prema primjeni eutektičkih otapala i inovativnih metoda u ekstrakciji polifenola iz komine grožđa. Eutektička otapala (DES) su nezapaljiva, viskozna i nehlapljiva otapala koja se istražuju kao zamjena konvencionalnim otapalima. Ultrazvučna ekstrakcija je metoda ekstrakcije kojom se smanjuje vrijeme ekstrakcije, potrošnja energije i povećava se prinos u odnosu na konvencionalne metode. U ovom radu provedena je ultrazvučna ekstrakcija komine grožđa pomoću NADES-a odabranog softverom COSMOtherm. Primijenjen je NADES betain:glukoza. Ukupni sadržaj polifenolnih spojeva u ekstraktima komine grožđa određen je pomoću Folin-Ciocalteau (FC) reagensa. Ispitan je utjecaj veličine čestica, sondi i način vođenja procesa na rezultate ekstrakcije. Provedena je i optimizacija procesnih parametara ekstrakcije pomoću Box-Behnkenovog plana pokusa.

Abstract (english)

In recent decades more attention has been directed towards the use of eutectic solvents and innovative methods in the extraction of polyphenols from grape pomace. Deep eutectic solvents (DES) are non-flammable, viscous and non-volatile solvents that are being investigated as alternatives for conventional solvents. Ultrasonic extraction is an extraction method that reduces extraction time, energy consumption and increases yield compared to conventional methods. In this work ultrasonic extraction of grape pomace was carried out using NADES selected by COSMOtherm software. NADES betain:glucose was applied. The total content of polyphenolic compounds in grape pomace was determined by Folin-Ciocalteau (FC) method. The influence of particle size, probes and the method of conducting the process on the extraction results was examined. The optimization of the extraction process parameters was also carried out using the Box-Behnken model.

• Keywords: eutektička otapala; ekstrakcija potpomognuta ultrazvukom; polifenolni spojevi; COSMOtherm; Box-Behneken model
• Keywords (english): deep eutectic solvents; ultrasound-assisted extraction; polyphenol compounds; COSMOtherm; Box-Behnken model
• Language: croatian
• URN:NBN: urn:nbn:hr:159:096939
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-27 13:18:59