Određivanje in vitro antioksidativnog kapaciteta, sadržaja polifenola i učinka na PMA-inducirano oksidativno izgaranje u ljudskim fagocitima odabranih ekstrakta povrća

master's thesis

• Title (english): Assesment of the in vitro antioxidant capacity, phenolic content and of the effect on PMA-induced oxidative burst in human phagocytes of selected vegetable extracts
• Author: Petra Kučan
• Mentor: Mauro Serafini (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Nutrition Science); Zagreb
• Defense date and country: 2014-02-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Oksidativni stres povezan je s nekoliko patoloških stanja kao što su kardiovaskularna, neurodegenerativna, rak, čak i starenje. Predloženo je da prehrana bogata antioksidansima korisno djeluje na ljudsko zdravlje, spriječavajući oksidacijske procese. Kako bi pronašli nove izvore snažnih antioksidansa istražili smo četiri različita ekstrakta povrća (divljih šparoga, crne repe te dva različita ekstrakta raštike). Sadržaj ukupnih fenola procijenjen je Folin-Ciocalteau metodom. Divlje šparoge pokazale su najviši udio polifenola, slijede raštika te crna repa. Neenzimski antioksidativni kapacitet povrća određen primjenom FRAP i TRAP metode pokazao je značajnu korelaciju sa sadržajem polifenola. S obzirom da je antioksidativni kapacitet procijenjen kemijskim metodama, rezultati se ne mogu projicirati na djelovanje istih uzoraka in vivo. Stoga se istražila sposobnost ekstrakta da moduliraju izazvano oksidativno izgaranje u ljudskim stanicama protočnom citometrijskom analizom. Šparoge su pokazale najjači kapacitet u modulaciji oksidativnog izgaranja u neutrofilima i monocitima sa značajnim pozitivnim učinkom ovisnim o dozi.

Abstract (english)

Oxidative stress is associated with several pathologies like cardiovascular, neurodegenerative, cancer and even aging. It has been suggested that a diet rich in antioxidants would be beneficial to human health, inhibiting oxidation processes. In order to find new sources of powerful antioxidants four different Croatian vegetable extracts were evaluated (wild asparagus, black radish and two different extracts of collard). The total phenolic content was assessed with Folin-Ciocalteau method. The evaluation of Non Enzymatic Antioxidant Capacity (NEAC) was carried out using FRAP and TRAP assays. Wild asparagus presented the highest phenolic content followed by collard species and black radish. NEAC values demonstrated significant correlation with phenolic content. Considering that antioxidant capacity obtained by chemical methods cannot extrapolate samples action in vivo we investigated the ability of extracts to modulate induced human cells oxidative burst by flow cytometric assay. Asparagus showed the greatest capacity in modulation of neutrophils and monocytes oxidative burst with significant positive dose dependent effect.

• Keywords: Povrće; Polifenoli; Antioksidativni kapacitet; Oksidativni stres; Oksidativno izgaranje u fagocitima
• Keywords (english): Vegetables; Polyphenols; Antioxidant capacity; Oxidative stress; Phagocytes´ oxidative burst
• Language: croatian
• URN:NBN: urn:nbn:hr:159:143465
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 09:13:22