Utjecaj visokog tlaka na ekstrakciju biološki aktivnih spojeva iz gloga

master's thesis

• Title (english): The effect of high pressure assisted extraction on isolation of bioactive compounds in hawthorn
• Author: Marija Majstorović
• Mentor: Verica Dragović Uzelac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2015-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Cilj rada je bio odrediti utjecaj visokog tlaka na ekstrakciju biološki aktivnih spojeva (ukupnih fenola, flavonoida, hidroksicimetnih kiselina i flavonola) iz lista i cvijeta gloga (Crataegi folium cum flore) pri čemu je ispitivan utjecaj sljedećih parametara: utjecaj vrste i polarnosti otapala (30 i 70 % -tne vodene otopine metanola i etanola), vremena trajanja ekstrakcije (3 i 10 minuta), te vrijednosti tlaka (300 i 500 MPa). Istraživanje je provedeno na sušenom listu i cvijetu gloga veličine čestica, d ≤ 500 mikrona. U ekstraktima dobivenim pri prethodno navedenim uvjetima određivani su ukupni fenoli, ukupni flavonoidi, ukupne hidroksicimetne kiseline i ukupni flavanoli, spektrofotometrijski. Budući da fenolni spojevi pridonose antioksidacijskoj aktivnosti u ekstraktima je određivan i antioksidacijski kapacitet DPPH i FRAP metodama. Prema dobivenim rezultatima, optimalni parametri pri kojima se ostvaruje najveći prinos fenolnih spojeva primjenom visokog tlaka 300-500 MPa i vremena trajanja ekstrakcije je 70%- tna vodena otopina etanola. Najveći prinos antioksidacijskog kapaciteta kod DPPH metode je dobiven primjenom 30% metanola, a kod FRAP-a podjednako visoke vrijednosti su dobivene primjenom metanola (70%) i etanola (30% i 70%). Vrijeme i tlak ekstrakcije nisu značano utjecali na prinos bioaktivnih spojeva.

Abstract (english)

The aim of this study was to determine the effect of high pressure assisted extraction on isolation of bioactive compounds (total phenols, total flavonoids, total hydroxicinnamic acid and total flavonols) from hawthorn leaf and flower herbal mixture (Crataegi folium cum flore) and these parameters were examined: effect of solvent type and polarity (aqueous methanol and ethanol), extraction time (3 and 10 min) and pressure values (300 and 500 MPa). The research was carried out with hawthorn dried leaf and flower with particles size d ≤ 500 microns. According to mentioned conditions in extracts, total phenolic, total flavonoid, total hydroxicinnamic acid and total flavonol content were determined by spectrophotometric method. Phenolic compounds contribute to antioxidant activity, so antioxidant capacity by DPPH and FRAP methods was determined. According to the results, it was found that optimal parameters for the highest yield of phenolic compounds at pressure 300-500 MPa and time 3-10 min was achieved by use of 70 % aqueous ethanol. The highest yield of antioxidant capacity determined by DPPH method was achieved by use of 30% aqueous methanol, while equaly high values were achieved by use of aqeous methanol (70%) and ethanol (30% i 70%), determined by FRAP method. At the same time, the extraction time and pressure values did not significantly affect the yield of bioactive compounds.

• Keywords: cvijet i list gloga; fenolni spojevi; visoki hidrostatski tlak; antioksidacijski kapacitet
• Keywords (english): hawthorn leaf and flower; phenolic compunds; high hydrostatic pressure; antioxidant capacity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:381871
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 12:48:49