Optimiranje sastava podloge za rast kvasca Scheffersomyces stipitis i proizvodnju S-adenozil-L-metionina

master's thesis

• Title (english): Optimization of medium composition for growth yeast Scheffersomyces stipitis and production of S-adenosyl-L-methionine
• Author: Maja Tuđan
• Mentor: Damir Stanzer (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2015-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

S-adenozil-L-metionin (SAMe) je biološki važna molekula, sudionik mnogih biokemijskih reakcija kod sisavaca. Poremećaji u njegovom metabolizmu povezani su s bolestima jetre te raznim neurološkim poremećajima. Budući da se SAMe koristi pri liječenju ovih oboljenja, važno je pronaći učinkovite načine njegove proizvodnje. Kvasac Scheffesomyces stipitis koristi se za proizvodnju etanola i mikrobne biomase, te za proizvodnju heterolognih proteina. U ovom radu uzgajana je biomasa soja M12 te divljeg soja kvasca S. stipitis na različitim podlogama s ciljem optimiranja proizvodnje SAMe. Divlji soj kvasca brže raste u odnosu na soj M12 u svim istraživanim podlogama, ali soj M12 proizvodi veće količine SAMe. Kod soja M12 nakon 48 sati u O-podlozi s dodatkom glicerola izmjerena je količina SAMe od 24,5 mgg-1 s.tv., dok je kod divljeg soja ona iznosila 12,2 mgg-1 s.tv. Također, uočeno je i da smanjenje volumena podloge, tj. povećanje aeracije povećava udio SAMe u biomasi soja M12 kvasca S. stipitis.

Abstract (english)

S-adenosyl-L-methionine (SAMe) is a biologicaly important molecule because is involved in several biochemical reactions of mammals. Abnormalities in its metabolism are recognized in liver disease and various neurological disorders. Since SAMe is used in the treatment of such conditions, it is important to find effective ways of its production. Yeast Scheffesomyces stipitis is used for the production of ethanol and microbial biomass and for production of heterologous proteins. In this work, wild S. stipitis strain and M12 strain were grown on different media with the aim of optimize the production of SAMe. The wild strain grows faster in comparison to the M12 strain in all tested media but the M12 strain produces higher amounts of SAMe. The content of SAM in the M12 strain was 24.5 mgg-1 dry weight while the wild strain was 12.2 mgg-1 dry weight after 48 hour of cultivation in O-medium with addition of glycerol. Also, it was observed that volume reducing of the medium (ie. increasing aeration) increases the amount of SAMe in the biomass of S. stipitis M12 strain.

• Keywords: S-adenozil-L-metionin; Scheffersomyces stipitis (stari naziv Pichia stipitis); glicerol
• Keywords (english): S-adenosyl-L-methionine; Scheffersomyces stipites (formerly known as Pichia stipitis); glycerol
• Language: croatian
• URN:NBN: urn:nbn:hr:159:185437
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 10:04:31