Proizvodnja lipida sa kvascem Trichosporon oleaginosus na glukozi i ksilozi uz limitaciju rasta izvorom dušika

master's thesis

• Title (english): Lipid production by yeast Trichosporon oleaginosus on glucose and xylose under nitrogen limitation of growth
• Author: Josipa Lisičar
• Mentor: Mirela Ivančić - Šantek (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2013-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Nakupljanje lipida u oleaginoznim mikroorganizmima započinje kada je jedan nutrijent u hranjivoj podlozi utrošen (najčešćeg izvor dušika), a izvor ugljika, koji je u suvišku, troši se za sintezu triacilglicerola. U ovom istraživanju proučavana je proizvodnja lipida sa kvascem Trichosporon oleaginosus na hranjivim podlogama s glukozom i/ili ksilozom kao izvorom ugljika uz različite omjere masa izvora ugljika i dušika (C/N). Najveći udjela lipida postignut je uz omjer masa C/N od 45 g g-1. Ksiloza se pokazala pogodnijim supstratom za proizvodnju lipida nego glukoza. Tijekom rasta u podlozi s glukozom i ksilozom kvasac redoslijedno troši izvore ugljika. Kada koncentracija glukoze padne ispod 5 g L-1, kvasac počinje rasti na ksilozi. Tijekom šaržnog uzgoja s pritokom supstrata postignut je vrlo visoki udjel lipida u stanici od 64,84 %, dok je produktivnost procesa iznosila 0,227 g L-1 h-1.

Abstract (english)

Lipid accumulation in oleaginous microorganisams begins when one nutrient from the medium (usually nitrogen) is exhausted but an excess of carbon is still assimilated by the cells and is converted into triacylglycerols. Production of lipid by yeast Trichosporon oleaginosus on the medium contained glucose and/or xylose as the carbon source was studied in this research. Optimum C/N ratio for lipid accumulation was 45 g g-1 with glucose as carbon source. Maximum lipid accumulation occurred in batch culture with xylose. In the medium containing glucose and xylose microogranism showed sequential utilization of substrates. When the concentration of glucose decreased below than 5 g L-1, yeast started to spend xylose as the source of carbon. Maximum lipid accumulation occurred in fed-batch culture with glucose reaching a level of 64.34 % of the biomass and productivity of 0.227 g L-1 h-1.

• Keywords: oleaginozni mikroorganizmi; mikrobni lipidi; Trichosporon oleaginosus; biodizel
• Keywords (english): oleaginous microorganism; microbial lipids; Trichosporon oleaginosus; biodiesel
• Language: croatian
• URN:NBN: urn:nbn:hr:159:692164
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-04 10:45:56