Izolacija mutanata kvasca Pichia stipitis rezistentnih na nistatin

master's thesis

• Title (english): Isolation nystatin -resistant mutants of yeast Pichia stipitis
• Author: Maja Krpan
• Mentor: Damir Stanzer (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2012-04-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

(S)-adenozil-L-metionin (SAMe) je važna molekula u metabolizmu svih živih stanica. Nedostatci kao i povećane količine SAMe u organizmu mogu uzrokovati razne poremećaje u organizmu, a terapija SAMe-om je prisutna u liječenju bolesti jetre, depresije i Alzheimerove bolesti. Stoga je od iznimne važosti pronaći što učinkovitije i jeftinije načine proizvodnje. Cilj ovog rada bio je mutirati divlji soj kvasca Pichia stipitis UV zračenjem, ispitati njegovo preživljavanje prilikom navedenog zračenja i pronaći UV-mutanta sa što većom rezistencijom na antibiotik nistatin i sa smanjenon tolerancijom na EtOH odnosno UVmutanta koji svojim metabolizmom proizvodi veću količinu SAMe nego divlji soj. Rezultati pokazuju da je doza zračenja od 120 J/m2 prikladna za proizvodnju mutanata UV-zračenjem, proizvedeni mutanti toleriraju veće koncentracije nistatina od divljeg soja te pokazuju različitu toleranciju na etanol.

Abstract (english)

(S)-Adenosyl-L-methionine (SAMe) is an important molecule in metabolism of all living cells. Absence and extortionate amount of this substance can cause different disfunctions in organism. SAMe is used in treatment of liver disease, depression and Alzheimer´s disease. Therefore it is very important to find more efficient ways of producing SAMe. The aim of this work was to apply UV irradiation on yeast Pichia stipitis, to examine its capability to survive and acquire yeast with better resistance to antibiotic nystatin and lower tolerance to ethanol, yeast that produce more SAMe. The results showed that the amount of 120 J/m2 UV irradiation is adequate for producing these mutants. Mutants of yeast tolerate higher concentrations of nystatin compared to unmutated yeast and show different tolerance to ethanol.

• Keywords: (S)-adenozil-L-metionin; UV mutacija; nistatin; etanol; Pichia stipitis
• Keywords (english): (S)-Adenosyl-L-methionine; UV mutation; nystatin; ethanol; Pichia stipitis
• Language: croatian
• URN:NBN: urn:nbn:hr:159:875374
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-08 10:26:23