Based on the main requirements for the selection of probiotic strains, their survival under conditions of the gastrointestinal tract (GIT), 4 strains of Lactobacillus species were selected for further research in the probiotic concept: Lactobacillus sucicola/uvarum/capillatus BGGO7-28, Lactobacillus pentosus/ paraplantarum /plantarum BGGO5 -3, Lactobacillus casei BGGO5-7 and Lactobacillus rhamnosus BGGO5-47. Bacterial strain BGGO5-3 showed particularly high level of survival at pH values of simulated gastric juice 2 an simulation of the small intestine juice. Sensitivity of selected probiotic strains to different groups of antibiotics was investigated to rule out the possibility of spreading antibiotic resistance. Coexistence test has proved the ability of selected probiotic strains to grow as a joint culture. The assimilation of various prebiotic substrates: mannitol, lactulose, raftilose and innulin was also examined. According to the results, the selected probiotic strains grow best with mannitol and lactulose as a carbon source. Microbial adhesion to solvents carried out with ethylacetate, hexane and chloroform has indicated cell surface properties of the tested probiotic strains. Assimilation of cholesterol with the selected strains was similar for all tested strains and was conducted with the addition of bile salts, as they are naturally present in the small intestine where the probiotic bacteria should assimilate cholesterol.