Proizvodnja rekombinantnog proteina u suspenzijskoj CHO liniji

undergraduate thesis

• Title (english): The production of recombinant proteins in suspensive CHO cell line
• Author: Magdalena Horvat
• Mentor: Igor Slivac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2016-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

CHO stanična linija pogodna je za korištenje u tehnologiji stanica jer se može genetički modificirati i može proizvoditi rekombinantne proteine u svrhu istraživanja te se uzgajati u suspenziji. Cilj ovog rada je bio ispitati učinak različitih sastava medija na rast stanične linije, utrošak glukoze te proizvodnja proteina. U ovom radu ispitivan je rast suspenzijske CHO stanične linije u mediju s 5 % seruma te 1% seruma te 4% sojinog hidrolizat. Najveću specifičnu brzinu rasta pokazao je medij s 1% seruma i 4% sojinog hidrolizata te je u tom uzorku zabilježena najveća potrošnja glukoze od 17,372 mM.

Abstract (english)

CHO cell line is suitable for use in cell culture technology due to its genetic modification that made them immortalized and suitable for production of recombinant proteins for research and therapeutic purposes. The cell line grow as either adherent or suspension culture. The aim of this research was to examine the effect of different medium component on the growth of cell lines, the consumption of glucose and on the protein production. In this research was examined growth of suspension of CHO cell line in medium containing 5% of serum and second suspension containing 4% of soy hydrolyzate and 1% of serum respectively. The maximum specific growth speed occured in medium containing 1% of serum and 4% of soy hydrolyzate but the same medium also showed highest glucose consumption rate of 17,372 mM.

• Keywords: CHO (Chinese Hamster Ovary) stanična linija; hidrolizat soje; vijabilnost stanica
• Keywords (english): CHO (Chinese Hamster Ovary) cell line; soy hydrolysate; cell viability
• Language: croatian
• URN:NBN: urn:nbn:hr:159:180922
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 08:02:11