Biološki aktivni spojevi liofilizirane pulpe i sjemenki kiwana (cucumis metuliferus)

undergraduate thesis

• Title (english): Biologically active compounds of freeze-dried pulp and seed of kiwano (cucumis metuliferus)
• Author: Ana Ilić
• Mentor: Verica Dragović Uzelac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2012-09-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Kiwano (Cucumis metuliferus) je biljka koja pripada porodici Cucurbitaceae te je njen plod bogat različitim biološkim aktivnim spojevima. Cilj ovog rada je bio odrediti koncentraciju ukupnih fenola, flavan-3-ola i antioksidacijski kapacitete u ekstraktima liofilizirane pulpe i sjemenki kiwana primjenom različitih otapala kako bi se ustvrdilo s kojim se postiže najbolji ekstrakcijski kapacitete te utvrditi postoji li korelacija između antioksidacijskog kapaciteta i koncentracije fenolnih spojeva. Određivanje ukupnih fenola i flavan-3-ola provedeno je spektrofotometrijskim metodama, a za određivanje antioksidacijskog kapaciteta se koristila FRAP metoda. Najbolji ekstrakcijski kapacitet izolacije ukupnih fenola iz liofilizirane pulpe i sjemenki ostvaren je primjeno 80%-tne vodene otopine etanola, dok za izolaciju flavan-3-ola iz liofilizirane pulpe i sjemenki kiwana je 80% vodena otopina acetona. Najveći antioksidacijski kapacitet u liofiliziranoj pulpi te sjemenkama kiwana određen je u ekstraktima dobivenim s 80% vodenom otopinom acetonitrila. Antioksidacijski kapacitet nije u korelaciji s koncentracijom fenolnih spojeva.

Abstract (english)

Kiwano (Cucumis metuliferus) is a plant that belongs to the family Cucurbitaceae and its fruit abundant variety of biologically active compounds. The aim of this study was to determine the concentration of total phenolics, flavan-3-ols and antioxidant capacity in extracts of freeze-dried pulp and seeds of kiwano using different solvents to determine with whom to obtain maximum extraction capacity and to determine if there is a correlation between the antioxidant capacity and concentration of phenolic compounds. Determination of total phenols and flavan-3-ol was carried out using spectrophotometric methods for the determination of antioxidant capacity by FRAP method was used. The best extraction capacity isolation of total phenols from freeze-dried pulp and seeds realized by applying 80% aqueous ethanol, and the isolation of flavan-3-ols from freeze-dried pulp and seeds of kiwano a 80% aqueous acetone. Highest antioxidant capacity of freeze-dried pulp and seeds of kiwano determined in extracts obtained with 80% aqueous acetonitrile. Antioxidant capacity was not correlated with the concentration of phenolic compounds.

• Keywords: Kiwano; Cucumis metuliferus; liofilizacija; polifenoli; biološki aktivni spojevi; antioksidacijski kapacitet
• Keywords (english): Kiwano; Cucumis metuliferus; freez-drying; polyphenols; biologically active compounds; antioxidation capacity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:357482
• Study programme: Title: Nutrition; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-15 09:32:45