| Abstract | Tijekom ovog istraživanja analiziran je 21 uzorak različitih vrsta meda hrvatskog podijetla (Varaždinska županija, sezona 2010) i to med kestena, med lipe, cvjetni i livadni med te uniflorni medovi facelije, kadulje i lavande. Određen je udjel ukupnih fenola modificiranom Folin-Ciocalteu metodom, te antioksidacijska aktivnost pomoću dvije metode, DPPH i FRAP. Sadržaj ukupnih fenola kretao se u rasponu 104,13–342,22 mg GAE/kg meda (kesten), 36,45–55,81 mg GAE/kg meda (lipa), 55,44–88,84 mg GAE/kg meda (cvjetni med), 67,81–136,62 mg GAE/kg meda (livadni med), 71,89–155,50 mg GAE/kg meda (uniflorni med); "radical scavenging" aktivnost izražena kao IC50 vrijednost, u rasponu 0,28–13,32 mg/mL (kesten), 17,13–18,19 mg/mL (lipa), 11,23–15,35 mg/mL (cvjetni med), 0,33–21,71 mg/mL (livadni med), 0,44–3,68 mg/mL (uniflorni med); a antioksidacijska aktivnost izražena kao FRAP vrijednost, u rasponu 3,78–35,21 μM Fe(II) 10% otopine meda (kesten), 4,24–30,08 μM Fe(II) 10% otopine meda (lipa), 7,40–12,39 μM Fe(II) 10% otopine meda (cvjetni med), 6,39–27,62 μM Fe(II) 10% otopine meda (livadni med), 8,19–31,17 μM Fe(II) 10% otopine meda (uniflorni med). |
| Abstract (english) | This paper analyzed 21 sample of different types of honey from Croatia (Varaždin, season 2010): chestnut honey, lime-tree honey, flower and meadow honey and unifloral honeys from facelije, sage and lavender. It was determined a phenolic content by modified Folin-Ciocalteu method, and antioxidant activity using two methods, DPPH and FRAP. The content of total phenols ranged 104,13-342,22 mg galic acid/kg of honey (chestnut), 36,45-55,81 mg galic acid/kg of honey (lime-tree) 55,44-88,84 mg galic acid/kg of honey (flower honey), 67,81-136,62 mg galic adic/kg of honey (meadow honey), 71,89-155,50 mg galic adic/kg of honey (unifloral honey); "radical scavenging" activity is expressed as an IC50 value in the range 0,28-13,32 mg/mL (maroon), 17,13-18,19 mg/mL (linden), 11,23-15,35 mg/mL (flower honey), 0,33-21,71 mg/mL (meadow honey), 0,44-3,68 mg/mL (unifloral honey) and antioxidant activity expressed as the FRAP value in the range 3,78-35,21 μM Fe(II) 10% solution of honey (chestnut) , 4,24-30,08 μM Fe(II) 10% solution of honey (lime-tree), 7,40-12,39 μM Fe(II) 10% solution of honey (flower honey), 6,39-27,62 μM Fe(II) 10% solution of honey (honey meadow) , 8,19-31,17 μM Fe(II) 10% solution of honey (unifloral honey). |
