Polifenoli sirovoga i jestivoga repičinoga ulja

undergraduate thesis

• Title (english): Polyphenols of crude and refined rapeseed oil
• Author: Lucija Roščić
• Mentor: Klara Kraljić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Oil and Fat Technology); Zagreb
• Defense date and country: 2016-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Repičino ulje je bogato polifenolnim spojevima koji posjeduju antioksidacijska svojstva tako što hvataju slobodne radikale te usporavaju reakcije oksidacije. Cilj ovog rada bio je ispitati mogućnost izolacije čistih polifenolnih spojeva, kanolola i fenilindana, iz sirovoga i jestivoga repičinoga ulja kako bi se mogli naknadno koristit za istraživanje njihovih svojstava. Polifenolni spojevi izolirani su metanolom i naknadno pročišćeni tankoslojnom kromatografijom. Procesom izolacije je iz 240 g sirovoga repičinoga ulja izolirano 26,57 mg kanolola pri čemu iskorištenje procesa iznosi 52,55%. Za izolaciju fenilindana je korišteno 720 g sirovoga repičinoga ulja te je izolirano 6,43 mg navedenog spoja. Iskorištenje procesa izolacije fenilindana iznosi 56,15%. Čistoća proizvedenih ekstrakata je visoka te za ekstrakt sirovog repičinog ulja iznosi 97,7% dok za jestivo repičino ulje iznosi 88,6%. Na temelju dobivenih vrijednosti se može zaključiti da se sirovo repičino ulje može koristiti za izolaciju kanolola, a jestivo repičino ulje za izolaciju fenilindana.

Abstract (english)

Rapeseed oil is rich in polyphenols, compounds with radical scavenging activity that can slow down oxidation reactions. The aim of this work was to determine a possibility of isolation of polyphenol compounds, canolol and phenylindane in crude and refined rapeseed oil. Methanol was used for extracion and thin-layer chromatography was used for purification. For isolation of canolol was used 240 g of crude rapeseed oil from which was isolated 26,57 mg of canolol. Process yield for canolol was 52,55%. For isolation of phenylindane was used 720 g of refined rapeseed oild from which was isolated 6,43 mg of phenylindane. Process yield for phenylindane was 56,15%. Purity of extracts was high; for crude rapeseed oil extract it was 97,7% and for refined rapeseed oil extract it was 88,6%. Based on the given data it can be concluded that crude rapeseed oil can be used for isolation of canolol and refined rapeseed oil can be used for isolation of phenylindane.

• Keywords: uljana repica; repičino ulje; polifenoli; kanolol; fenilindan
• Keywords (english): rapeseed; rapeseed oil; polyphenols; canolol; phenylindane
• Language: croatian
• URN:NBN: urn:nbn:hr:159:465103
• Study programme: Title: Nutrition; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 10:42:26