Određivanje alergena kikirikija u uzorcima krem proizvoda i čokolada ELISA metodom

master's thesis

• Title (english): Determination of peanut allergens in cocoa cream and chocolate by ELISA method
• Author: Mladena Zeljko
• Mentor: Ksenija Marković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Quality Control); Zagreb
• Defense date and country: 2016-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Tijekom ovog istraživanja određeni su potencijalni alergeni kikirikija u osam uzoraka krem proizvoda i osam uzoraka čokolada s lješnjakom pomoću ELISA (engl. Enzyme-Linked ImmunoSorbent Assay) metode te je ispitan stav potrošača o navodima potencijalne prisutnosti alergena kikirikija na deklaraciji tih proizvoda. Samo u jednom od analiziranih uzoraka nisu detektirani alergeni kikirikija, dok je u ostalima određen udio u rasponu od 0,27 ± 0,010 do 4,05 ± 0,030 mgkg-1. Između analiziranih uzoraka u kojima su određeni alergeni kikirikija, samo šest sadrži navod o potencijalnoj prisutnosti alergena kikirikija na deklaraciji. Ispitivanjem stavova potrošača o navodima na deklaraciji krem proizvoda i čokolada utvrđeno je da navod "Može sadržavati kikiriki u tragovima" 59 % ispitanika izdvaja kao najpoznatiji. Tijekom ovog istraživanja, u sedam analiziranih uzoraka krem proizvoda te dva uzorka čokolada s lješnjakom bez navoda, ELISA metodom detektirani su alergeni kikirikija.

Abstract (english)

In this study the commercial ELISA (Enzyme-Linked ImmunoSorbent Assay) test was used for determination of potential peanut allergens in eight samples of cocoa cream and eight samples of chocolate with hazelnut, and also the consumers opinion toward advisory labelling was examined. One sample showed no traces of peanut while in the rest of samples peanut allergens were determined in the range from 0,27 ± 0,010 to 4,05 ± 0,030 mgkg-1. Among analysed samples in which peanut allergens were determined, only six of them had advisory labelling. 59 % of examined consumers are most familiar with advisory label: "May contain peanuts in traces". In this study, in seven samples of cocoa cream and two samples of chocolate with hazelnut, which had no advisory label, peanut allergens were detected by ELISA method.

• Keywords: alergeni; kikiriki; ELISA; čokolada; krem proizvod
• Keywords (english): allergens; peanut; ELISA; chocolate; cocoa cream
• Language: croatian
• URN:NBN: urn:nbn:hr:159:529552
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-25 11:18:00