Proteinski izolati iz uljnih pogača lana i konoplje-priprava, karakterizacija i biološka aktivnost

master's thesis

• Title (english): Protein isolates from flaxseed and hempseed cake - preparation, characterization and biological activity
• Author: Petra Bosanac
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2017-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Posljednjih nekoliko desetljeća prisutan je porast korištenja organskih ostataka nakon prerade proizvoda iz različitih sektora poljoprivrede i industrije. Veliki potencijal primjene imaju uljne pogače koje zaostaju nakon ekstrakcije ulja i danas se uglavnom koriste kao stočna hrana ili gnojivo. Pogače lana i konoplje sadrže različite komponente koje se smatraju vrlo važnim za ljudsko zdravlje, a značajan dio predstavljaju proteini koji mogu imati potencijal u tehnologiji životinjskih stanica. U ovom radu provedena je priprava proteinskih izolata lana i konoplje iz dva različita uzorka pri čemu je dobiven udio proteina u rasponu 43,7-60,42% za lan, odnosno 27,7-48,12% za konoplju. Ispitan je i utjecaj različitih koncentracija izolata na na proliferaciju HEK 293T stanične linije prilikom čega je utvrđeno da izolat lana nije stimulirao rast HEK 293T stanica u odnosu na standardni medij s 5% seruma,a izolat konoplje je pokazao inhibicijski učinak na rast stanica.

Abstract (english)

There has been an increased exploitation of organic residues from various sectors of agriculture and industries over the past few decades. Oil cakes, that remain after oil extraction from seeds, are mainly used as a feed source or fertilizer. Flaxseed and hempseed meal, the most interesting oil cakes today, contain various components considered to be of great importance to human health and a significant portion of proteins that can be used in animal cell technology.This thesis discusses various preparation of flax and hemp protein isolates in two different samples, whereby the protein fraction was obtained in the range of 43.7-60.42% in flax and 27.7-48.12% in the hemp protein isolate. In addition to flaxseed and hempseed meal, human HEK 293T cell line derived from the transformation of human embryonic kidney cells with DNA adenovirus 5 was also used. The influence of different isolate concentrations on the proliferation of HEK 293T cell line was determined, whereby the growth of HEK 293T cells was not stimulated by flax protein isolate in compare to the standard medium with 5% serum, and also, a hemp protein isolate showed an inhibitory effect on cell growth.

• Keywords: uljne pogače lana i konoplje; proteiski izolat; udio proteina; proliferacija stanica
• Keywords (english): flaxseed and hempseed meal; protein isolates; protein share; cell growth
• Language: croatian
• URN:NBN: urn:nbn:hr:159:740097
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-16 10:41:11