| Abstract | Motar je aromatska halofilna biljka koja se zbog svog bogatog sastava biološki aktivnih spojeva koristi u prehrani. U ovom radu istraživan je utjecaj različitih vrsta otapala (voda, etanol (96%), etanol-voda (1:1), aceton (100%), aceton-voda (1:1), metanol (100%), metanol-voda (1:1), etanol-metanol-aceton (1:1:1) i etanol-aceton-metanol-voda (1:1:1:1)) na ekstrakciju fenolnih spojeva iz lista motar. Ekstrakcija je provedena refluksiranjem pri temperaturi ključanja otapala kroz 1 h. U dobivenim ekstraktima provedeno je spektrofotometrijsko (UV/VIS) određivanje masenih udjela ukupnih fenola, flavonoida, hidroksicimetnih kiselina i flavonola. Dobiveni rezultati su pokazali da primjena ekstrakcijskog otapala ima utjecaj na izolaciju fenolnih spojeva te da se bolji učinak ekstrakcije fenolnih spojeva postiže dodatkom vode u otapalo, nego primjenom čistog otapala, a vodena otopina etanola ima najbolji ekstrakcijski učinak kod izolacije ukupnih fenola (77,05 mg/g), hidroksicimetnih kiselina (53,49 mg/g) i flavonola (38,24 mg/g), dok je primjena čistog otapala, odnosno metanola, pokazala najbolji učinak kod ukupnih flavonoida (38,13 mg/g). Provedeno istraživanje pokazuje da je list motar dobar izvor fenolnih spojeva. |
| Abstract (english) | Sea fennel is aromatic halophyte which have high content of biologically active compounds and it’s used in nutrition. In this thesis was examine how different solvents effect (water, ethanol (96%), ethanol-water (1:1), acetone (100%), acetone-water (1:1), methanol (100%), methanol-water (1:1), ethanol-acetone-methanol (1:1:1) and ethanol-acetonemethanol- water (1:1:1:1)) on extraction phenolic compounds of sea fennel leaves. Duration of extraction was 1 hour on boiling temperature of each solvent. Extracts were analysed with spectrophotometry (UV/VIS) for total phenols, flavonoids, hydroxycinnamic acid and flavonols content. Results show that solvent has effect on extraction of phenolic compounds, solvents mixed with water have higher capacity to extract phenolics than pure solvents and ethanolwater has the highest capacity of extraction of mass fraction of total polyphenols (77,05 mg/g), hydroxycinnamic acids (53,49 mg/g) and flavonols (38,24 mg/g), and pure solvent, methanol, has the highest capacity of extraction of total flavonoids (38,13 mg/g). These results show that leaves of sea fennel have a high phenolic content. |
