Određivanje rezidua cefalosporina u mišiću goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS)

master's thesis

• Title (english): Determination of cephalosporin residue in cattle, pigs, poultry and fish muscle using the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS)
• Author: Ana Oguić
• Mentor: Blaženka Kos (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2018-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Neadekvatna uporaba antibiotika u humanoj i veterinarskoj medicini dovodi do razvoja antimikrobne rezistencije, širenja otpornosti mikroorganizma te pojave njihovih ostataka u namirnicama životinjskog porijekla. Jedna od najčešće primijenjenih vrsta antibiotika su cefalosporini. Da bi ih adekvatno identificirali i kvantificirali bilo je potrebno razviti metodu i ispitati utjecaj metode za identifikaciju rezidua cefalosporina u mišićima goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS), što je ujedno i glavni cilj ovog rada. Također, validirana metoda za određivanje cefalosporina u mišićima različitih životinjskih vrsta visoke je specifičnosti, točnosti i preciznosti te zadovoljava kriterije o provođenju analitičkih metoda i tumačenju rezultata prema važećoj Uredbi komisije 2002/657/EZ. Metoda je primijenjena u rutinskoj analizi na Hrvatskom veterinarskom institutu. Na prisustvo 9 najčešćih cefalosporina testirana su 42 uzorka pri čemu nije pronađen ni jedan pozitivan uzorak.

Abstract (english)

Inadequate use of antibiotics in human and veterinary medicine leads to the development of antimicrobial resistance, the spread of microorganism resistance and the appearance of their residues in food of animal origin. One of the most frequently used types of antibiotics are cephalosporins. In order to identify and quantify, it was necessary to develop a method and to examine the effect of the method for identifying the residual cephalosporin residues in cattle, pigs, poultry and fish muscles using ultra-high performance liquid chromatography and high resolution mass spectrometry (UHPLC/Q-TOF-MS), which is also the main objective of this paper. Validated method for the determination of cephalosporin in the muscles of different animal species is of high specificity, accuracy and precision and meets the criteria for the conduct of analytical methods and interpretation of results under the current Commission Regulation 2002/657 / EC. The method was applied in a routine analysis at the Croatian Veterinary Institute. In the presence of 9 most common cephalosporins, 42 samples were tested and no positive samples were found

• Keywords: cefalosporini; UHPLC; Q-TOF; MS
• Keywords (english): cephalosporin; UHPLC; Q-TOF; MS
• Language: croatian
• URN:NBN: urn:nbn:hr:159:928719
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-11-26 13:52:34