In vitro biološka svojstva hidrolizata proteina iz pogače konoplje

master's thesis

• Title (english): In vitro biological activity of protein hydrolysates obtained from hempseed meal
• Author: Martina Bagović
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2018-09-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Industrijska konoplja (Canabis sativa L.) se uz ostale primjene koristi i u proizvodnji ulja, pri čemu nakon ekstrakcije ulja iz sjemenki zaostaje vrijedan nusproizvod – pogača, koja obiluje proteinima. Enzimskom hidrolizom proteina izdvojenih iz uljnih pogača dobivaju se peptidi koji posjeduju određenu biološku aktivnost koja se može iskoristiti za proizvodnju prirodnih lijekova i dodataka prehrani. Stoga je cilj ovog rada bio ispitati biološka svojstva hidrolizata proteina konoplje u in vitro uvjetima. Antiproliferacijski učinak hidrolizata ispitan je na dvije tumorske stanične linije (HeLa i MCF-7) ovisno o korištenim enzimima tijekom hidrolize (enzimi alkalaza, neutraza i protamex) te veličini peptidnih frakcija hidrolizata proteina dobivenih enzimom alkalazom (>10, 3-10 i <3 kDa). Dobiveni rezultati ukazuju da svi pripravljeni hidrolizati posjeduju antioksidacijsku aktivnosti kao i pripravljene frakcije hidrolizata. Antiproliferacijsku aktivnost na tumorskim staničnim linijama pokazao je samo hidrolizat dobiven alkalazom te njegove veće frakcije (>10 i 3-10 kDa). Također je dokazan i protektivni učinak hidrolizata i frakcije (<3 kDa) proteina konoplje dobivenih pomoću enzima protamex na inducirani oksidacijski stres u HeLa stanicama.

Abstract (english)

Industrial hempseed (Canabis sativa L.) among its diverse applications can also be used in the oil industry. After obtaining oil from hempseed, remains a valuable by product – hempseed meal, which has high levels of protein. Peptides with biological activity are released by enzymes hydrolysis of hempseed meal proteins. This potential can be used for the production of natural medications or food supplements. Therefore, in this thesis, the biological activity of protein hydrolysates from hempseed meal was studied in vitro. Antiproliferative effects were studied in two cancer cell lines (HeLa and MCF-7) in dependence of enzyme used for hydrolysis (Alcalase, Neutrase and Protamex) and the size of peptide fractions obtained by Alcalase (>10, 3-10 and <3 kDa). Study has confirmed the antioxidative activity of all prepared hydrolysates and their peptide fractions. The antiproliferative activity was observed only in hydrolysates obtained by Alcalase and its bigger fractions (>10 and 3-10 kDa). Lastly, the protective effect of hydrolysate obtained by Protamex and its fraction (<3 kDa) during induced oxidative stress was confirmed in HeLa cell line.

• Keywords: pogača konoplje; hidrolizati proteina; antioksidacijska aktivnost; oksidacijski stres
• Keywords (english): hempseed meal; protein hydrolysate; antioxidative activity; oxidative stress
• Language: croatian
• URN:NBN: urn:nbn:hr:159:019872
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-11-27 10:43:59