Antioksidacijska aktivnost i ukupni fenoli ekstrakata medicinske gljive Ganoderma lucidum

master's thesis

• Title (english): Antioxidant activity and total phenolics of medicinal mushroom Ganoderma lucidum extracts
• Author: Veronika Radišić
• Mentor: Sunčica Beluhan (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2019-12-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Gljiva Ganoderma lucidum je izvor bioaktivnih sastojaka, kao što su triterpeni, polisaharidi, steroidi, fenolni sastojci i glikoproteini. Polisaharidi, posebice -glukani, triterpeni i fenoli posjeduju antioksidacijska svojstva. U ovom radu su proučavani udjeli ukupnih fenolnih spojeva i antioksidacijska aktivnost u vodenim i metanolnim ekstraktima micelija i plodišta (bazidiokarpa) gljive G. lucidum. Za dokazivanje antioksidacijskog kapaciteta ekstrakata, korištene su tri temeljne metode određivanja: DPPH, ABTS i FRAP. Metanolni ekstrakt je pokazao maksimalnu sposobnost vezanja DPPH slobodnih radikala (EC50 = 0,29 mg/mL) i ABTS+ (EC50 = 0,58 mg/mL), dok je u vodenom ekstraktu izmjerena bolja sposobnost vezanja željeznih iona (EC50 = 5 μg/mL). Udjel ukupnih fenolnih spojeva i antioksidacijski kapacitet bio je značajno veći u metanolnim ekstraktima (p < 0,01). Uočena je pozitivna korelacija između koncentracije ukupnih fenola i antioksidacijske aktivnosti. Rezultatima ovog istraživanja je potvrđeno da i micelij i plodište gljive G. lucidum posjeduju osobita antioksidacijska svojstva, te se mogu koristiti kao izvrsni izvor supstancija s antioksidacijskom aktivnošću za terapeutsku primjenu.

Abstract (english)

Fungi of Ganoderma lucidum genus contain bioactive components such as triterpenes, polysaccharides, steroids, phenolic compounds and glycoproteins. Polysaccharides, particularly - glucans, triterpenes and phenolic compounds have antioxidant properties. In this work, the total phenolic contents and antioxidant activity of water and methanolic extracts of G. lucidum in mycelia and mature fruiting body (basidiocarp) were evaluated. Three complementary assays, DPPH, ABTS and FRAP were used to screen the antioxidant capacity of extracts. Both aqueous and methanolic extracts were analyzed for free radical scavenging activity. Methanolic extract showed maximum scavenging activity for DPPH (EC50 = 0.29 mg/mL) and ABTS+ (EC50 = 0.58 mg/mL), whereas aqueous extract had better scavenging for ferric reducing antioxidant power (EC50 = 5 μg/mL). Total phenolic content and total antioxidant capacity were significantly higher in methanolic extract (p < 0.01). A positive correlation existed between the phenolic content and antioxidant activity. Results indicated that both mycelia and mature fruiting body of G. lucidum possess remarkable antioxidant properties, which can be used as excellent sources of antioxidant compounds for therapeutic application.

• Keywords: medicinske gljive; Ganoderma lucidum; bioaktivni sastojci; vezanje slobodnih radikala; antioksidacijski kapacitet
• Keywords (english): Medicinal mushrooms; Ganoderma lucidum; Bioactive compounds; Free radical scavenging; Antioxidant capacity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:746820
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-31 09:25:03