Utjecaj prirodnih eutektičkih otapala na aktivnost i skladišnu stabilnost imobilizirane i slobodne lipaze

master's thesis

• Title (english): Natural deep eutectic solvent impact on activity and storage stability of immobilized and free lipase form
• Author: Mia Radović
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2020-01-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Implementacija načela zelene kemije upotrebom prirodnih eutektičkih otapala (NADES) u biokatalizi omogućuje unaprijeđenje procesa kroz bolju supstratnu topljivost, poboljšanu enzimsku aktivnost i stabilnost. U ovom radu utvrđuje se najpovoljnije otapalo koje može poslužiti kao reakcijski i skladišni medij za slobodnu (slLip) i imobiliziranu (imLip) C. antarctica lipazu B. U tu svrhu ispitana su tri NADES-a (ChEG, ChGly, ChGlc) sa različitim udjelima vode (10, 30 i 50 % (v/v)). Inkubacija enzima u svim otapalima 28 dana pri 25 °C, pokazala je nadmoćniju stabilnost i aktivnost imLip u odnosu na slLip. Dok slLip zadržava maksimalno 40 % rezidualne aktivnosti nakon 28 dana inkubacije (u ChGlc30%), aktivnost imLip pada sporije, pri čemu u istom periodu zadržava i do 67 % aktivnosti (u ChGly10%). Zaključno, ChGly izdvaja se kao NADES s najvećim potencijalom da postane stabilizirajući medij za lipaze.

Abstract (english)

Implementation of green chemistry principles through the use of natural deep eutectic solvents (NADES) in biocatalysis can improve reaction efficiency and sustainability through better substrate solubility, improved enzyme activity and stability. To determine the best solvent to act as reaction and stabilization medium for immobilized (imLip) and free (slLip) C. antarctica lipase B, three NADES (ChEG, ChGly, ChGlc) with different water shares (10, 30 i 50 % (w/w)) have been assessed. Enzyme incubation in these solvents at 25 °C during the period of 28 days has shown greater activity and stability of imLip in regard to the slLip. While slLip maintains up to 40 % residual activity after 28 day incubation period (in ChGlc30%), imLip keeps a significantly higher value in the same period, up to 67% (in ChGly10%). Finally, ChGly steps out as the best NADES that could potentially serve as lipase stabilizing medium.

• Keywords: zelena kemija; prirodna eutektička otapala; lipaza; biokataliza; skladična stabilnost
• Keywords (english): green chemistry; natural deep eutectic solvents; lipase; biocatalysis; storage stability
• Language: croatian
• URN:NBN: urn:nbn:hr:159:990611
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-13 12:32:18