Formulacija i karakterizacija inkapsuliranih sustava liposoma s dopaminom iz kore banane

undergraduate thesis

• Title (english): Formulation and characterization of liposome systems encapsulated with dopamine from banana peel
• Author: Jakov Cvetković
• Mentor: Draženka Komes (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Chemistry and Technology of Carbohydrates and Confectionery Products); Zagreb
• Defense date and country: 2020-09-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U posljednje vrijeme agro-industrijski otpad plijeni pozornost znanstvenika zbog visokih udjela bioaktivnih sastojaka, posebice polifenolnih spojeva, čija konzumacija je povezana s brojnim pozitivnim učincima na ljudsko zdravlje. U ovome radu, kora banane, tropskog voća iz porodice Musaceae, izabrana je kao sekundarni biljni materijal za ekstrakciju polifenolnih spojeva primjenom konvencionalnih (infuzija, dekokcija, maceracija) i inovativnih tehnika ekstrakcije (ekstrakcija potpomognuta... More ultrazvukom, ekstrakcija potpomognuta mikrovalovima, ekstrakcija subkritičnom vodom). U svrhu karakterizacije dobivenih ekstrakata određeni su udjeli ukupnih polifenola, udio dopamina i antioksidacijski kapacitet te je ekstrakt s najboljim bioaktivnim parametrima inkapsuliran u liposome. Formuliranim liposomima određena je inkapsulacijska učinkovitost i fizikalno-kemijski parametri (veličine, zeta potencijala i indeksa polidisperzije). Infuzija (80 °C, 30 min) se pokazala najuspješnijom tehnikom u ekstrakciji ukupnih polifenola (25,38 mg EGK gˉ¹ s.tv. uzorka), ali je dekokt (100 °C, 20 min) zbog najizraženijih antioksidacijskih svojstava (0,196 i 0,164 mmol Trolox gˉ¹ s.tv. uzorka) i najvišeg udjela dopamina (12,63 mg EGK gˉ¹ s.tv. uzorka) inkapsuliran u liposome. Inkapsulacijska učinkovitost dopamina bila je u rasponu od 35,10 do 41,12 %, a izmjerene vrijednosti fizikalno-kemijskih parametara ukazuju na relativnu homogenost i stabilnost formuliranih liposoma. Less

Abstract (english)

In recent years, agro-industrial waste has been subject of many scientific research due to its high content of bioactive compounds, especially polyphenols, whose consumption is linked with various positive effects on human health. In this thesis, banana peel, part of a tropic fruit from the Musaceae family, has been chosen as a secondary plant material for the extraction of polyphenolic compounds using conventional (infusion, decoction, maceration) and innovative extraction methods... More (ultrasound-assisted extraction, microwave-assisted extraction, subcritical water extraction). Obtained extracts were characterized by determining the total polyphenols content, dopamine content and antioxidant capacity, and the extract with the best bioactive parameters was further used for encapsulation in liposomes. Formulated liposomes were characterized by determining the encapsulation efficiency and physico-chemical parameters. Infusion (80 °C, 30 min) showed to be most successful technique in the extraction of total polyphenols (25,38 mg EGK gˉ¹ dmb), and decoction (100 °C, 20 min) in dopamine extraction (12,63 mg EGK gˉ¹ dmb). Extract obtained by decoction was also characterized with the highest antioxidant capacity (0,196 and 0,164 mmol Trolox gˉ¹ dmb) and was further used for encapsulation in liposomes. Encapsulation of banana peel’s extract resulted in a relatively low encapsulation efficiency of dopamine (35,10 – 41,12 %). Measured values of size, zeta potential and polydispersion index indicate the relative homogeneity and uniformity of the formulated liposomes. Less

• Keywords: ekstrakcija; inkapsulacija; kora banane; liposomi; polifenoli
• Keywords (english): extraction; encapsulation; banana peel; liposomes; polyphenols
• Language: croatian
• URN:NBN: urn:nbn:hr:159:316564
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-10-16 12:25:34