| Abstract | U ovom radu ispitan je utjecaj lokacije i klimatskih uvjeta na udio bioaktivnih spojeva u listu i stabljici samonikle koprive ubrane s 14 lokacija u RH s područja Ozlja i okolice, Zagreba, Žumberka, Ogulina, Like i Istre. Liofilizirani uzorci ekstrahirani su ubrzanom ekstrakcijom otapalima pri povišenom tlakom (ASE) pri prethodno definiranim optimalnim uvjetima ekstrakcije: a) za uzorke lista - temperatura 110 °C, statičko vrijeme ekstrakcije 10 minuta i 4 ciklusa ekstrakcije, i b) za uzorke stabljike - temperatura 80 °C te statičko vrijeme ekstrakcije 5 minuta i 4 ciklusa ekstrakcije. U dobivenim ekstraktima spektrofotometrijski je određen udio ukupnih fenola, klorofila a, klorofila b, ukupnih klorofila i ukupnih karotenoida. Prema dobivenim rezultatima, list koprive sadržavao je znatno više udjele analiziranih spojeva u odnosu na stabljiku. Najviši udio ukupnih fenola u listu određen je u uzorku s područja Ozlja i okolice (1162,95±36,33 mg GAE 100 gˉ¹), dok je najviši udio ukupnih fenola u stabljici određen u uzorku s područja Istre (245,39±3,33 mg GAE 100 gˉ¹). Najviši udjeli ukupnih klorofila u listu i stabljici određeni su u uzorcima s područja Istre (list 1110,24±11,15 mg 100 gˉ¹; stabljika 72,34±2,39 mg 100 gˉ¹), kao i najviši udio ukupnih karotenoida (list 148,59±5,53 mg 100 gˉ¹; stabljika 17,50±0,59 mg 100 gˉ¹). |
| Abstract (english) | In this work, the influence of location and climatic conditions on the content of bioactive compounds in the leaf and stem of wild nettle harvested from 14 locations in the Republic of Croatia from Ozalj area, Zagreb, Žumberak, Ogulin, Lika and Istria was examined. Lyophilized samples were extracted using accelerated solvent extraction (ASE) under the previously optimized extraction conditions: a) for leaf samples - temperature 110°C, static extraction time 10 minutes and 4 extraction cycles, and b) for stem samples - temperature 80°C, static extraction time 5 minutes and 4 extraction cycles. In obtained extracts, content of total phenols, chlorophyll a, chlorophyll b, total chlorophylls and total carotenoids was determined spectrophotometrically. According to the obtained results, nettle leaf contained considerably higher content of analyzed compounds when compared to the stem. The highest content of total phenols in the leaf was determined in the sample from the Ozalj area (1162.95±36.33 mg GAE 100 gˉ¹), while the highest total phenols in the stem were determined in the sample from Istria (245.39±3.33 mg GAE 100 gˉ¹). The highest total chlorophylls in leaf and stem were determined in samples from Istria (leaf 1110.24±11.15 mg 100 gˉ¹; stem 72.34±2.39 mg 100 gˉ¹), as well as the highest content of total carotenoids (leaf 148,59±5.53 mg 100 gˉ¹, stem 17.50±0.59 mg 100 gˉ¹). |
