Razvoj metode za određivanje makrolida, tetraciklina i sulfonamida u medu metodom tekućinske kromatografije s masenom detekcijom

master's thesis

• Title (english): Method development for detection of macrolides, tetracyclines and sulfonamides in honey with liquid chromatography with mass detection
• Author: Ana Pinčar
• Mentor: Mirjana Hruškar (mentor)
• Committee member: Blaženka Kos (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Quality Control); Zagreb
• Defense date and country: 2020-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Sve veća prisutnost antibiotika u humanoj, ali i veterinarskoj medicini, dovela je do potrebnih mjera kojima se rezidue antibiotika moraju kontrolirati posebice u matriksima kao što je hrana jer predstavljaju izravnu prijetnju zdravlju potrošača. Od toga nije izuzet ni med kao prehrambeni proizvod izrazitog nutritivnog sastava, a za kojeg nema definiranih NDK (najmanja dopuštena količina) vrijednosti. Najzastupljeniji antibiotici u medu pokazali su se makrolidi, tetraciklini te sulfonamidi te je stoga posvećena pozornost njima u ovome radu, pri čemu je proveden razvoj metode prikladne za istovremeno određivanje svih već spomenutih vrsta antibiotika. Eksperimentom je utvđeno da je metoda tekućinske kromatografije sa masenom spektrometrijom (LC-MS/MS) prikladna za određivanje analita iz skupine makrolida, tetraciklina i sulfonamida.

Abstract (english)

Wide usage of antibiotics in human and veterinary medicine brought more focus on monitoring of antibiotic residues in matrices as food due to potential risk for consumer’s health. Honey, with a range of nutritional value, is also being monitored despite no MRL (maximum residue levels) were established. Macrolides, tetracyclines and sulfonamides are most common antibiotics in honey, therefore the focus in this experiment was to develop a method suitable for detection of all the antibiotics above. After performed experiment, it is determined that liquid chromatography with mass spectrometry (LC-MS/MS) is suitable method for multiclass determination of analytes from group of macrolides, tetracyclines and sulfonamides.

• Keywords: antibiotici; masena spektrometrija; med; Quechers; tekućinska kromatografija
• Keywords (english): antibiotics; honey; liquid chromatography; mass spectrometry; Quechers
• Language: croatian
• URN:NBN: urn:nbn:hr:159:372291
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-01-20 11:24:39