Endogeni enzimi ploda masline

master's thesis

• Title (english): Endogenous enzymes in olive fruit
• Author: Angela Vujnović
• Mentor: Dubravka Škevin (mentor)
• Committee member: Sandra Balbino (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Oil and Fat Technology); Zagreb
• Defense date and country: 2021-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Provedene su ekstrakcije enzima β-glukozidaze i lipoksigenaze iz pulpe i tijesta maslina hrvatske sorte Oblica smrznutih na dva različita temperatura reţima (-20 °C i -80 °C). Ispitivanje aktivnosti β-glukozidaze se odreĎivalo spektrofotometrijski na 405 nm, a lipoksigenaze na 234 nm. Spektrofotometrijska analiza nije pokazala aktivnost β-glukozidaze u tijestu i pulpi, dok je najveća aktivnost lipoksigenaze zabiljeţena u uzorcima tijesta smrznutih na -20 °C. Ispitivanje ovisnosti aktivnosti β-glukozidaze iz badema (Prunus Dulcis) o varijacijama koncentracija p-nitrofenil glukopiranozida je rezultiralo u Michaelis Menten krivuljom s Km 18,1204 mmol Lˉ¹ i Vmax 3,6152 μmol mLˉ¹ minˉ¹. Ovisnost aktivnosti sojine lipoksigenaze (Glycine Max) o varijacijama koncentracija linolenske masne kiseline je rezultiralo Michaelis Menten krivuljom s Km 6,2800 mmol Lˉ¹ i Vmax od 5,599 μmol mLˉ¹ minˉ¹, dok s linolnom nije pokazalo Michaelis Menten krivulju. Aktivnost β-glukozidaze je proporcionalna s koncentracijom enzima, dok se kod lipoksigenaze nije uspjela utvrditi ovisnost bez obzira na supstrat.

Abstract (english)

Extraction of β-glucosidase and lipoxygenase from pulp and paste of Croatian olive cultivar Oblica frozen at two different temperature regimes (-20 °C i -80 °C). β-glucosidase activity assay was determined at 405 nm and lipoxygenase activity at 234 nm. Spectrophotometric analysis did not determine β glucosidase activity, while the highest lipoxygenase activity was determined in olive paste frozen at -20°C. A study of dependence of almond β-glucosidase (Prunus Dulcis) activity on variations of p-nitrophenil glucopiranoside concentrations resulted in Michaelis Menten curve with Km 18,1204 mmol Lˉ¹ i Vmax 3,6152 μmol mLˉ¹ minˉ¹ . Dependence of soy bean lipoxygenase (Glycine Max) activity on variations of linolenic acid resulted in Michaelis Menten curve with Km 6,2800 mmol Lˉ¹ i Vmax 0,5504 μmol/mL min, while on variation of linoleic acid concentrations did not result in Michaelis Menten curve. β glucosidase acitivity is proportional to the enzyme concentration, while lipoxygenase acitivity dependence was not determined regardless of the substrate

• Keywords: pulpa masline; maslinovo tijesto; Oblica; β-glukozidaza; lipoksigenaza
• Keywords (english): olive pulp; olive paste; Oblica; β-glucosidase; lipoxygenase
• Language: croatian
• URN:NBN: urn:nbn:hr:159:406696
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-27 12:48:52