Određivanje antioksidacijskog kapaciteta i udjela šećera u češnjaku

master's thesis

• Title (english): Determination of antioxidant capacity and sugar content in garlic
• Author: Tvrtko Karlo Kovačević
• Mentor: Mirjana Hruškar (mentor)
• Committee member: Marina Krpan (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Quality Control); Zagreb
• Defense date and country: 2021-11-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Češnjak je vrlo pogodan za istraživanja jer se razmnožava nespolno, odnosno radi se o klonovima. Unatoč navedenom, među njima postoji razlika u sastavu i količini antioksidansa i šećera. Antioksidansi su od velike važnosti u prehrani ljudi jer reduciraju slobodne radikale i prihvaćaju nesparene elektrone te na taj način čuvaju sastavne dijelove stanica poput lipida i DNK. Kako bi se doznalo koje namirnice imaju više ili manje antioksidansa te samim time veći ili manji učinak na zdravlje potrebno odrediti udio antioksidansa te njihov kapacitet. Za analizu je prikupljeno 77 ekotipova češnjaka s područja Republike Hrvatske kao i izvan njenih granica. Šećeri imaju važnu ulogu u staničnim funkcijama kao što je pohranjivanje energije te su od interesa istraživačima, pogotovo inulin koji se nalazi u malom broju biljaka, pri čemu je češnjak jedan od njih. Detekcija i kvantifikacija šećera u uzorcima češnjaka provedena je pomoću tekućinske kromatografije visoke djelotvornosti.

Abstract (english)

Garlic is suitable for research because it breeds inexofield, that is, they are clones. Despite the above, among them there is a difference in the composition and amount of antioxidants and sugars. Antioxidants are of great importance in human nutrition because they preserve the constituent parts of cells such as lipids and DNA. To find out which foods have more or less antioxidants and therefore a greater or lesser effect on health, it is necessary to determine the proportion of antioxidants and their capacity. For the analysis, 77 garlic ecotypes from the Republic of Croatia were collected as well as outside its borders. Sugars play an important role in cellular functions such as energy storage and are of interest to researchers, especially inulin found in a small number of plants, where garlic is one of them. Sugar detection and quantification in garlic samples was carried out using high-performance liquid chromatography.

• Keywords: antioksidacijski kapacitet; češnjak; ekotip; HPLC; šećeri
• Keywords (english): antioxidant capacity; ecotype; garlic; HPLC; sugars
• Language: croatian
• URN:NBN: urn:nbn:hr:159:845537
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-11-16 11:43:47