Priprema polifenolnih ekstrakata iz otpada vinske industrije u niskotemperaturnim eutektičkim otapalima uz primjenu softvera COSMOtherm

master's thesis

• Title (english): Polyphenolic extracts preparation from wine industry waste in natural deep eutectic solvents using COSMOtherm software
• Author: Nikolina Martić
• Mentor: Ivana Radojčić Redovniković (mentor)
• Committee member: Karin Kovačević-Ganić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2021-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Ekstrakti spremni za direktnu primjenu koji sadrže sekundarne biljne metabolite idealni su za primjenu u brojnim industrijama s obzirom na sve veće tendencije znanstvenika prema zelenoj ekstrakciji. Otapala pogodna za njihovu pripremu su prirodna niskotemperaturna eutektička otapala (NADES), a kao polazni materijal moguće je iskoristiti otpad vinske industrije poput komine grožđa, bogate polifenolima. U ovom radu pripravljeni su ekstrakti komine bijelog grožđa u etanolu i NADES-ima odabranima pomoću softvera COSMOtherm. Ekstraktima je određen ukupni sadržaj polifenola Folin-Ciocalteu reagensom, ORAC vrijednosti te su identificirani i kvantificirani najzastupljeniji polifenolni spojevi. Najveći prinos ekstrakcije imao je NADES betain:glukoza te je njegova bioaktivnost ispitana in vitro na Caco-2 staničnoj liniji pomoću MTS metode i DCFH-DA metode. COSMOtherm se dokazao kao pouzdan način predviđanja topljivosti spojeva u željenim otapalima te se betain:glukoza pokazao kao dostojna zamjena etanolu u postupku ekstrakcije iz biljnih materijala. Također je utvrđena prisutnost katehina, epikatehina, epigalokatehina te rutin trihidrata u komini grožđa. Optimalne koncentracije polifenola za antioksidativni i citotoksični učinak moraju još biti dalje istraživane i utvrđene kako bi polifenolni ektrakti bili učinkoviti i sigurni za ljudsku primjenu.

Abstract (english)

Ready-to-use extracts that contain secondary plant metabolites are ideal for use in many industries given the scientists' growing tendencies towards green extractions. Solvents suitable for their preparation are natural deep eutectic solvents (NADES), and as a starting material it is possible to use wine industry waste, such as grape pomace, rich in polyphenols. In this study, extracts of white grape pomace were prepared in ethanol and NADES selected by COSMOtherm. Extracts total polyphenols content was determined with Folin-Ciocalteu reagent, ORAC values were also determined and prevalent polyphenolic compounds were identified and quantified. Betaine: glucose provided the best extraction efficiency and its biological activity was evaluated in vitro on the Caco-2 cell line using the MTS and the DCFH-DA assay. COSMOtherm has proven to be reliable in predicting the solubility of compounds in given solvents, and betaine: glucose extract has proven to be a good alternative to ethanol in the extraction process. The presence of catechin, epicatechin, epigallocatechin and rutin trihydrate in grape pomace was also shown. Optimal concentrations of polyphenols in extracts for antioxidant and cytotoxic effects need to be further evaluated to be effective and safe for human consumption.

• Keywords: niskotemperaturna eutektička otapala; polifenoli; COSMOtherm; komina grožđa
• Keywords (english): natural deep eutectic solvents; polyphenols; COSMOtherm; grape pomace
• Language: croatian
• URN:NBN: urn:nbn:hr:159:751196
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-12-01 10:50:21