Analiza adsorpcijskih i desorpcijskih procesa bioaktivnih molekula, proteina i glukoze s alginatnih mikrokapsula

master's thesis

• Title (english): Analysis of the adsorption and desorption processes of bioactive molecules, proteins and glucose on alginate microcapsules
• Author: Izvorka Laljak
• Mentor: Maja Benković (mentor)
• Committee member: Davor Valinger (predsjednik povjerenstva)
• Committee member: Ivana Radojčić Redovniković (član povjerenstva)
• Committee member: Mirjana Čurlin (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Process Engineering); (Section for Fundamental Engineering); Zagreb
• Defense date and country: 2022-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj ovog rada bio je analizirati i usporediti procese adsorpcije i desorpcije proteina (albumin izoliran iz goveđeg seruma, engl. bovine serum albumin, BSA), ugljikohidrata (glukoza) i polifenola (galna kiselina kao modelna molekula) s alginatnih mikrokapsula. Procesi adsorpcije i desorpcije zatim su opisani matematičkim modelima kako bi se definirali kinetički parametri procesa i utvrdili dominantni mehanizmi koji su odgovorni za adsorpciju i desorpciju. Usporedbom vrijednosti konstanti brzina adsorpcije (k) i početnih brzina adsorpcije (h0) dobivenih primjenom modela pseudodrugog reda, najviše vrijednosti zabilježene su za BSA, nakon čega slijede galna kiselina i glukoza. Analizom vrijednosti konstante brzine desorpcije (k), najviše su vrijednosti zabilježene za galnu kiselinu, koju slijedi glukoza i BSA. Za procese adsorpcije glukoze i galne kiseline, najreprezentativniji model je pseudodrugi red, što sugerira da je limitirajući korak kemijska sorpcija tvari, te da brzina adsorpcije ne ovisi o koncentraciji adsorbata, nego o kapacitetu adsorbensa. Proces desorpcije pokretan je pseudo- Fickovim difuzijskim mehanizmom.

Abstract (english)

The objective of this thesis was to analyze and compare the adsorption and desorption processes of proteins (bovine serum albumin, BSA), carbohydrates (glucose) and polyphenols (gallic acid as a model molecule) on alginate microcapsules. The adsorption and desorption processes were then described by mathematical models in order to define the kinetic parameters of the process and determine the dominant mechanisms responsible for adsorption and desorption. By comparing the values of adsorption rate constant (k) and initial adsorption rate (h0) obtained using the pseudo-secondorder model, the highest values were recorded for BSA, followed by gallic acid and glucose. By analyzing the values of the desorption rate constant (k), the highest values were recorded for gallic acid, followed by glucose and BSA. For the adsorption processes of glucose and gallic acid, the most representative model was pseudo-second-order, which suggests that the limiting step of the process is the chemical sorption of substances, and that the rate of adsorption does not depend on the adsorbate concentration, but on the capacity of the adsorbent. The desorption process is characterized by the pseudo-Fickian diffusion mechanism.

• Keywords: adsorpcija; desorpcija; kinetički modeli; alginat; mikroinkapsulacija
• Keywords (english): adsorption; desorption; kinetic models; alginate; microencapsulation
• Language: croatian
• URN:NBN: urn:nbn:hr:159:705705
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-02-28 13:08:57