Antimikrobna i antioksidacijska aktivnost ferocenskih peptida dobiveni iz ferocen-1,1'-diamina

master's thesis

• Title (english): Antimicrobial and antioxidant activity of ferrocene peptides derived from ferrocene 1,1 diamine
• Author: Miriam Cikron
• Mentor: Jasna Mrvčić (mentor)
• Committee member: Monika Kovačević (predsjednik povjerenstva)
• Committee member: Lidija Barišić (član povjerenstva)
• Committee member: Damir Stanzer (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2022-07-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

U literaturi je prisutno sve više podataka o antimikrobnoj rezistenciji, posebice o rezistenciji na više lijekova koji se koriste u terapiji protiv gram-pozitivnih i gram-negativnih bakterija. Budući da su multirezistentni bakterijski sojevi sve rašireniji i brojniji, u cilju zaštite zdravlja važno je osigurati nove, učinkovite antimikrobne agense. Shodno tome, istražuju se potencijalni antimikrobni peptidi s ugrađenom ferocenskom jedinicom. U ovom je radu za osamnaest sintetiziranih ferocenskih peptida ispitana potencijalna antimikrobna i antioksidativna aktivnost. Antimikrobna aktivnost određena je disk difuzijskom metodom, metodom razrjeđivanja bujona i metodom brojanja kolonija, a u svrhu istraživanja antioksidacijske aktivnosti korištene su FRAP i DPPH metode. Biokonjugatima Ac-L-Ala-D-Pro-NH-Fn-NH-D-Pro-Boc (4) i Ac-D-Ala-D-Pro-NH-Fn-NH-L-Pro-Boc (5) iz skupine ferocenskih peptida IIIc, koji su pokazali antimikrobno djelovanje disk difuzijskom metodom prema test mikroorganizmima: S. aureus, E. faecium, P. aeruginosa i E. coli, određena je minimalna inhibitorna koncentracija prema navedenim bakterijskim vrstama i iznosi > 2 mM.

Abstract (english)

There is increasing evidence in the literature of antimicrobial resistance, often resistance to multiple drugs used in the therapy against gram-positive and gram-negative bacteria. As multidrug-resistant bacterial strains become more widespread and numerous, it is important to provide new, effective antimicrobial agents tp protect health. Potential antimicrobial agents are ferrocene peptides, and their biological evaluation is investigated in this work. Biological evaluation of eighteen synthesized ferrocene peptides was performed by determining their potential antimicrobial and antioxidant activities. The antimicrobial activity was studied by the disk diffusion method, broth dilution method and colony counting method, while the antioxidant activity was determined by the FRAP and DPPH. The bioconjugates Ac-L-Ala-D-Pro-NH-Fn-NH-D-Pro-Boc (4) and Ac-D-Ala-D-Pro-NH-Fn-NH-L-Pro-Boc (5) from the group of ferrocene peptides IIIc, showed antimicrobial activity by the disk diffusion method according to test microorganisms: S. aureus, E. faecium, P. aeruginosa and E. coli. The minimum inhibitory concentration was determined according to these bacterial species and is > 2 mM.

• Keywords: antimikrobna rezistencija; ferocenski peptidi; biološka evaluacija; antimikrobna aktivnost; antioksidacijska aktivnost
• Keywords (english): antimicrobial resistance; ferrocene peptides; biological evaluation; antimicrobial activity; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:648454
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-06-02 10:54:11