Analiza stabilnosti modelnog proteina lizozima u niskotemperaturnim eutektičkim otapalima na bazi različitih osmolita

master's thesis

• Title (english): Analysis of the stability of the model protein lysozyme in deep eutectic solvents based on different osmolytes
• Author: Marija Karin
• Mentor: Marina Cvjetko Bubalo (mentor)
• Committee member: Ivana Radojčić Redovniković (predsjednik povjerenstva)
• Committee member: Ana Tušek Jurinjak (član povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2023-12-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Sve je veća zabrinutost i potreba za pronalaskom manje štetnih otapala zbog sve veće zagađenosti okoliša pa su znanstvenici sve više usmjereni prema proučavanju i korištenju niskotemperaturnih eutektičkih otapala (engl. deep eutectic solvents, DES) koja su zanimljiva zbog jednostavne pripreme, niske cijene te niske ili gotovo nikakve toksičnosti. Najnovije istraživanje stvara poveznicu između DES-ova i osmolita, koji su male organske molekule koje stanice nakupljaju kao odgovor na okolišne stresove te imaju jako dobra stabilizirajuća svojsta. Ova nova otkrića pružaju izvrsnu priliku za projektiranje novih otapala i sustava na bazi osmolita koji su izravno inspirirani prirodnim mikrookruženjem biomakromolekula. Različite kombinacije osmolita tvore višekomponentne eutektičke sustave koji pomažu u održavanju nativne konformacije i funkcionalnosti proteina i drugih biomolekula u nepovoljnim uvjetima. U ovom radu je ispitan utjecaj niskotemperaturnih eutektičkih otapala na bazi osmolita (osmoDES) na toplinski induciranu agregaciju te stabilnost modelnog proteina lizozima pri ekstremnim temperaturama. Rezultati ukazuju na značajan potencijal primjene pripravljenih osmoDES-ova kao tekućeg skladišnog medija za proteine, s obzirom na njihove izuzetne stabilizacijske sposobnosti.

Abstract (english)

There is a growing concern and the need to find less harmful solvents due to increasing environmental pollution, so scientists are increasingly directed towards the study and use of deep eutectic solvents (DES), which are interesting due to their simple preparation, low cost and low or almost no toxicity. The latest research creates a link between DESs and osmolytes, which are small organic molecules that cells accumulate in response to environmental stress and have very good stabilizing properties. These new discoveries provide an excellent opportunity to design new osmolyte-based solvents and systems that are directly inspired by the natural microenvironment of biomacromolecules. Different combinations of osmolytes form multicomponent eutectic systems that help maintain the native conformation and functionality of proteins and other biomolecules under adverse conditions. In this study, the influence of DES based on osmolytes (osmoDES) on heat-induced aggregation and stability of the model protein lysozyme at extreme temperatures was examined. The results indicate the significant potential of using the prepared osmoDES as a liquid storage medium for proteins, given their exceptional stabilization capabilities.

• Keywords: niskotemperaturna eutektička otapala; osmoliti; stabilizacija
• Keywords (english): eep eutectic solvents; osmolytes; stabilization
• Language: croatian
• URN:NBN: urn:nbn:hr:159:282617
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2024-04-02 09:06:10