Protektivni i toksični učinci resveratrola - mehanizmi djelovanja na staničnoj razini

undergraduate thesis

• Title (english): Protective and toxic effects of resveratrol - mechanisms of action at the cellular level
• Author: Sofija Krotin
• Mentor: Ivana Kmetič (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2023-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

U ovom radu dan je kratki pregled potencijalih protektivnih i toksičnih učinaka resveratrola, s naglaskom na regulaciji stanične smrti i posljedičnom antikancerogenom djelovanju. U eksperimentalnom dijelu rada ispitana je mogućnost indukcije autofagije resveratrolom u CHO-K1 staničnoj liniji. Nakon 48 sati inkubacije s resveratrolom (5, 20, 50 i 100 µM) metodom protočne citometrije određen je udio stanica u autofagiji. Utvrđeno je da resveratrol ima statistički značajan učinak na indukciju autofagije pri koncentraciji 50 µM (p<0,001) u odnosu na kontrolni uzorak (stanice netretirane resveratrolom), dok pri koncentraciji 20 µM i 100 µM nije uzrokovao značajno odstupanje. Provedenim eksperimentalnim istraživanjem preliminarno je utvrđeno da resveratrol, ovisno o primijenjenoj dozi, inducira autofagiju u CHO-K1 staničnoj liniji, a pregled literature ukazao je da resveratrol posjeduje veliki potencijal uporabe kao farmakoterapeutik u prevenciji i liječenju određenih vrsta karcinoma, kardiovaskularnih bolesti, pretilosti i dijabetesa te neurodegenerativnih bolesti poput Alzheimerove, Parkinsonove ili Huntingtonove bolesti.

Abstract (english)

This study provides an overview of the potential protective and toxic effects of resveratrol, with an emphasis on the regulation of cell death and anticancer activity. In the experimental part of this research autophagy induction by resveratrol was analysed in CHO-K1 cell line. The proportion of cells in autophagy was determined by flow cytometry after 48 hours of incubation with resveratrol (5, 20, 50 and 100 µM). Compared to the control sample (cells not treated with resveratrol), statistically significant induction of autophagy was detected at concentration of 50 µM (p<0.001), while at concentrations of 20 µM and 100 µM resveratrol did not cause a significant alteration. This study preliminarily determined that resveratrol induces autophagy-mediated ovarian (CHO-K1) cell death in dose-dependent manner and literature overview confirmed great potential for use of resveratrol as a pharmacotherapeutic in prevention and treatment of certain types of cancer, cardiovascular diseases, obesity, diabetes and neurodegenerative diseases such as Alzheimer's, Parkinson's or Huntington's disease.

• Keywords: resveratrol; biološka aktivnost; terapeutski potencijal; autofagija; toksičnost
• Keywords (english): resveratrol; biological activity; therapeutic potential; autophagy; toxicity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:907588
• Study programme: Title: Nutrition; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-04-16 12:05:50