Utjecaj utišavanja klatrina na prijenos signala MAP kinaznog puta i prepoznavanje infekcije pomoću proteina MyD88 i IFI16 nakon infekcije adenovirusom tipa 26

master's thesis

• Title (english): Effect of clathrin knockdown on MAP kinase signaling pathway and recognition of infection by proteins MyD88 and IFI16 after adenovirus type 26 infection
• Author: Maša Lač
• Mentor: Ksenija Durgo (mentor)
• Mentor: Dragomira Majhen (komentor)
• Mentor: Isabela Drašković (pomoć pri izradi, neposredni voditelj)
• Committee member: Marina Cvjetko Bubalo (predsjednik povjerenstva)
• Committee member: Dragomira Majhen (član povjerenstva)
• Committee member: Ivana Radojčić Redovniković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2023-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Adenovirusni vektori se koriste u genskoj terapiji, terapiji tumora i razvoju cjepiva. Zbog visoke seroprevalencije najčešće korištenih adenovirusnih vektora, sve je više istraživanja usmjereno prema drugim adenovirusima, poput adenovirusa tipa 26 (HAdV26), koji je zbog niske seroprevalencije kandidat s visokim potencijalom za primjenu kao vektorsko cjepivo. U ovom je radu ispitana uloga klatrina u prijenosu signala MAP kinaznog puta nakon infekcije HAdV26 praćenjem aktivacije kinaza ERK i p38, kao i u prepoznavanju infekcije pomoću proteina MyD88 i IFI16. Nije zabilježena aktivacija kinaze ERK nakon infekcije HAdV26, dok je aktivacija p38 uočena u staničnoj liniji s najmanjom ekspresijom klatrina. Nakon infekcije HAdV26 uočen je unutarstanični transport proteina MyD88 i IFI16, što upućuje na njihovu uključenost u prepoznavanju infekcije HAdV26.

Abstract (english)

Adenoviral vectors are used in gene therapy, cancer therapy and vaccine development. Due to high seroprevalence of the most frequently used adenovirus vectors, recent research is directed towards other adenoviruses, such as adenovirus type 26 (HAdV26), which is a candidate with great potential for vaccine vector due to its low seroprevalence. In this work, the role of clathrin in MAP kinase pathway signalling after HAdV26 infection was examined by monitoring activation of ERK and p38 kinases, as well as in the recognition of infection by MyD88 and IFI16 proteins. No activation of ERK kinase was detected after HAdV26 infection, while p38 activation was observed in the cell line with the lowest clathrin expression. After HAdV26 infection, intracellular transport of proteins MyD88 and IFI16 was observed, indicating their role in recognition of HAdV26 infection.

• Keywords: adenovirus tip 26; utišavanje klatrina; MAP kinazni put; MyD88; IFI6
• Keywords (english): adenovirus type 26; clathrin silencing; MAP kinase pathway; MyD88; IFI16
• Language: croatian
• URN:NBN: urn:nbn:hr:159:900941
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-12 09:06:52