Usporedba sastava crijevne mikrobiote u ovisnosti o obrascu prehrane

master's thesis

• Title (english): Comparison of the composition of the gut microbiota in dependence on the dietary type
• Author: Hana Lač
• Mentor: Ivana Rumora Samarin (mentor)
• Mentor: Jurica Žučko (pomoć pri izradi, neposredni voditelj)
• Committee member: Jurica Žučko (predsjednik povjerenstva)
• Committee member: Irena Keser (član povjerenstva)
• Committee member: Zvonimir Šatalić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Chemistry and Biochemistry); Zagreb
• Defense date and country: 2023-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Crijevna mikrobiota definira se kao zajednica mikroorganizama koji koloniziraju gastrointestinalni trakt domaćina. Na sastav crijevne mikrobiote utječu brojni egzogeni i endogeni čimbenici, među kojima je prehrana jedan od ključnih. Prehrana bazirana na namirnicama biljnog podrijetla, koju karakterizira visok unos vlakana, sve je češći odabir zbog pozitivnog utjecaja na zdravlje, a pokazuje i potencijal za modulaciju sastava crijevne mikrobiote. Cilj ovog rada bio je povezati sastav crijevne mikrobiote s vrstom prehrane ispitanika. U istraživanju je sudjelovalo 59 ispitanika oba spola, od kojih 48 svejeda, 6 vegana te 2 vegetarijanca, koji su ispunili opći upitnik o životnim i prehrambenim navikama te upitnik o usklađenosti prehrane s mediteranskom. Sastav crijevne mikrobiote određen je sekvenciranjem varijabilnih regija V3 i V4 gena koji kodira za 16S rRNA. Podaci dobiveni sekvenciranjem obrađeni su u QIIME2 programu. Prema rezultatima ovog rada nije uočena značajna povezanost vrste prehrane i sastava crijevne mikrobiote na promatranoj skupini ispitanika.

Abstract (english)

The gut microbiota is defined as a community of microorganisms that colonize the host's gastrointestinal tract. The composition of the intestinal microbiota is influenced by several exogenous and endogenous factors, among which nutrition is one of the key ones. A plant-based diet, characterized by high fiber intake, is gaining popularity due to its positive impact on health, and shows the potential for modulating the composition of the gut microbiota. The aim of this study was to connect the composition of the microbiota with the subjects’ dietary type. The study included 59 subjects of both sexes, of which 48 omnivores, 6 vegans and 2 vegetarians, who completed a general questionnaire on lifestyle and eating habits and a questionnaire on the compliance of diet with the Mediterranean diet. The composition of the gut microbiota was determined by sequencing of variable regions V3 and V4 of the gene coding for 16S rRNA. The data obtained by sequencing was processed using QIIME2. According to the results of this study, there was no significant correlation between the dietary type and the composition of the gut microbiota in the observed group of subjects.

• Keywords: sastav crijevne mikrobiote; vrsta prehrane; biljna prehrana; zdravlje
• Keywords (english): gut microbiota composition; dietary type; plant-based nutrition; health
• Language: croatian
• URN:NBN: urn:nbn:hr:159:386557
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-12 09:16:51