Adhezijska svojstva bakterija mliječne kiseline izoliranih iz majčinog mlijeka

master's thesis

• Title (english): Adhesion properties of lactic acid bacteria isolated from human breast milk
• Author: Jana Hrsan
• Mentor: Andreja Leboš Pavunc (mentor)
• Mentor: Krešo Bendelja (komentor)
• Mentor: Nina Čuljak (pomoć pri izradi, neposredni voditelj)
• Mentor: Željko Cvetić (pomoć pri izradi, neposredni voditelj)
• Committee member: Blaženka Kos (predsjednik povjerenstva)
• Committee member: Marina Cvjetko Bubalo (član povjerenstva)
• Committee member: Ksenija Durgo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2023-07-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj ovog rada bio je odrediti mogu li se sojevi Levilactobacillus brevis MB1, MB2, MB13, MB20 i Limosilactobacillus fermentum MC1, izolirani iz majčinog mlijeka, potencijalno koristiti kao probiotici. Ispitana je uloga S-proteina i egzopolisaharida bakterijskih sojeva u adheziji i kompetitivnoj ekskluziji bakterije Salmonella enterica serovar Typhimurium FP1, primjenom Caco-2 stanične linije. Adhezijska sposobnost istih sojeva također je potvrđena ispitivanjem vezanja sojeva na lektinu sličan protein ZG16. Ispitivanjem imunomodulacijskog učinka izoliranih S-proteina i egzopolisaharida te sojeva producenata, korištenjem Caco-2 stanične linije tretirane s lipopolisaharidima porijeklom iz bakterije Escherichia coli i hTNF-α, pokazano je njihovo inhibitorno djelovanje na proizvodnju proupalnih citokina IL-6, IL-8, IL-1β i TNF-α.

Abstract (english)

The aim of this work was to determine whether strains of Levilactobacillus brevis MB1, MB2, MB13, MB20 and Limosilactobacillus fermentum MC1, isolated from breast milk, could be used as potential probiotics. The role of S-proteins and exopolysaccharides of bacterial strains on intestinal epithelial Caco-2 cell line adhesion and competitive exclusion of the bacterium Salmonella enterica serovar Typhimurium FP1 was tested. The adhesion ability of the same strains was also confirmed by examining the binding of the strains to the lectin-like protein ZG16. The immunomodulatory effect of strain specific isolated S-proteins or exopolysaccharides and live bacteria using Caco-2 cells treated with lipopolysaccharides from Escherichia coli and human TNF-α was assesed. Inhibition of proinflammatory cytokines IL-6, IL-8, IL-1β and TNF-α production was demonstrated.

• Keywords: probiotici; bakterije mliječne kiseline; adhezija; S-proteini; egzopolisaharidi
• Keywords (english): probiotics; lactic acid bacteria; adhesion; S-proteins; exopolysaccharides
• Language: croatian
• URN:NBN: urn:nbn:hr:159:309711
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-21 09:05:31